Banerjee A, Richiert D M, Bagchi M
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201.
Biochim Biophys Acta. 1991 Mar 8;1077(1):56-64. doi: 10.1016/0167-4838(91)90525-5.
The polypeptides of vitreous humor, aqueous humor and iris-ciliary complex cells of eyes were phosphorylated with [gamma-32P]ATP without exogenous protein kinase. Phosphorylated polypeptides were analyzed by sodium dodecyl sulfate gel electrophoresis and autoradiography. The phosphorylated polypeptides of rabbit vitreous humor showed many high molecular weight prominent bands, but no detectable phosphoproteins were found in the 12 kDa or lower range. Bovine vitreous humor has predominantly acidic polypeptides and some of them are below 20 kDa. Rabbit and bovine iris-ciliary complex and rabbit aqueous humor showed a prominent common 4 kDa phosphopolypeptide which could also be synthesized by cloned populations of cells from the bovine iris and the rabbit iris-ciliary body. It is possible that the 4 kDa phosphopolypeptide of the aqueous humor is synthesized by the iris-ciliary complex cells.
在无外源蛋白激酶的情况下，用[γ-32P]ATP对眼玻璃体液、房水和虹膜睫状体复合体细胞的多肽进行磷酸化。磷酸化多肽通过十二烷基硫酸钠凝胶电泳和放射自显影进行分析。兔玻璃体液的磷酸化多肽显示出许多高分子量的明显条带，但在12 kDa及以下范围未发现可检测到的磷蛋白。牛玻璃体液主要含有酸性多肽，其中一些低于20 kDa。兔和牛的虹膜睫状体复合体以及兔房水显示出一条突出的共同4 kDa磷酸化多肽，该多肽也可由来自牛虹膜和兔虹膜睫状体的克隆细胞群体合成。房水的4 kDa磷酸化多肽有可能是由虹膜睫状体复合体细胞合成的。
