Faria T N, Ogren L, Talamantes F, Linzer D I, Soares M J
Department of Physiology, University of Kansas Medical Center, Kansas City 66103.
Biol Reprod. 1991 Feb;44(2):327-31. doi: 10.1095/biolreprod44.2.327.
The purpose of this investigation was to identify the cellular origin of placental lactogen-I (PL-I) expression in the mouse placenta and to cytologically define the transition from PL-I to PL-II expression during gestation. PL-I mRNA expression was assessed by in situ hybridization, and expression of PL-I and PL-II protein was determined by immunocytochemical analysis. PL-I mRNA and protein were localized to trophoblast giant cells. Trophoblast giant cells ceased producing PL-I at midgestation and began expressing PL-II. PL-I immunoreactivity was present in trophoblast giant cells on Days 9 and 10 of gestation but was not detectable in trophoblast giant cells on Day 11 of gestation. Immunoreactive PL-II-producing giant cells were detected first on Day 10 of gestation, continuing on Day 11 of gestation. Expression of PL-I and PL-II signals a significant functional transition in trophoblast giant cells of the developing mouse placenta.
本研究的目的是确定小鼠胎盘中胎盘催乳素-I（PL-I）表达的细胞起源，并从细胞学角度界定妊娠期PL-I向PL-II表达的转变。通过原位杂交评估PL-I mRNA表达，并通过免疫细胞化学分析确定PL-I和PL-II蛋白的表达。PL-I mRNA和蛋白定位于滋养层巨细胞。滋养层巨细胞在妊娠中期停止产生PL-I，并开始表达PL-II。PL-I免疫反应性在妊娠第9天和第10天的滋养层巨细胞中存在，但在妊娠第11天的滋养层巨细胞中未检测到。产生免疫反应性PL-II的巨细胞在妊娠第10天首次检测到，并在妊娠第11天持续存在。PL-I和PL-II的表达标志着发育中小鼠胎盘滋养层巨细胞的重大功能转变。
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