Laboratorio de Biorregulación, Unidad de Morfofisiología, FES Iztacala, UNAM, Tlalnepantla, Estado de México.
Pancreas. 2010 Mar;39(2):193-200. doi: 10.1097/MPA.0b013e3181c156d9.
To investigate the protective role of steroid hormones on streptozotocin (STZ)-induced apoptosis in rat pancreatic beta cells.
Two sets of experiments were performed. In the first, male rats were orchidectomized and substituted 72 hours later with testosterone, estradiol, or progesterone, and 24 hours later, administered with STZ. Subjects were killed 6 hours later, and apoptosis was determined in sections of the pancreas. In the second experiment, male or female rats were gonadectomized, were further substituted with testosterone, and then administered STZ. Six hours later, the animals were killed, and apoptosis, as well as immunoreactive expression of insulin, catalase, or Cu/Zn superoxide dismutase, was determined in sections of the pancreas. In addition, gonadectomized male or female subjects were substituted with testosterone and administered STZ, and 24 hours later, serum glucose and insulin were measured.
It was found that the cytoprotective effect was only shown in testosterone-treated male rats but not progesterone- or estradiol-treated male rats. In addition, the effect was seen in male rats but not in female rats, and there was an inverse correlation between apoptotic index and antioxidant enzyme immunoreactivity.
The cytoprotective effect of testosterone is sex specific and is related to the induction of antioxidant enzyme activities in pancreatic beta cells.
研究甾体激素对链脲佐菌素（STZ）诱导的大鼠胰岛β细胞凋亡的保护作用。
进行了两组实验。在第一组实验中，雄性大鼠接受睾丸切除术，并在 72 小时后用睾酮、雌二醇或孕酮替代，24 小时后给予 STZ。6 小时后处死动物，检测胰腺切片中的细胞凋亡。在第二组实验中，雄性或雌性大鼠接受睾丸切除术，并用睾酮进一步替代，然后给予 STZ。6 小时后处死动物，检测胰腺切片中的细胞凋亡以及胰岛素、过氧化氢酶或 Cu/Zn 超氧化物歧化酶的免疫反应性表达。此外，还对接受睾丸切除术的雄性或雌性动物用睾酮替代并给予 STZ，24 小时后测量血清葡萄糖和胰岛素。
结果发现，只有经睾酮处理的雄性大鼠表现出细胞保护作用，而经孕酮或雌二醇处理的雄性大鼠则没有。此外，这种作用仅见于雄性大鼠，而不见于雌性大鼠，并且凋亡指数与抗氧化酶免疫反应性呈负相关。
睾酮的细胞保护作用具有性别特异性，与胰岛β细胞中抗氧化酶活性的诱导有关。
