Department of Clinical Nutrition, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Dig Dis Sci. 2010 Oct;55(10):2814-20. doi: 10.1007/s10620-009-1101-0. Epub 2010 Jan 22.
Parenteral nutrition (PN)-induced liver injury is associated with gut atrophy, and probiotics have demonstrated the ability to stabilize the intestinal microecosystem and offer protection against bacterial translocation from the gut to the liver. Therefore, we hypothesized that enteral Bifidobacterium supplements could alleviate PN-associated liver injury.
Three-week-old New Zealand rabbits were divided into three groups: control, PN, and PN + Bif group (PN plus enteral feeding 0.5 × 10(8) Bifidobacterium adolescentis per day). After 10 days, serum levels of liver enzyme and endotoxin were measured, and histology of liver and ileum were performed. Blood and homogenized samples of tissue from the mesenteric lymph nodes, lung, and spleen were cultured for detecting bacteria translocation. Intestinal permeability was determined by sugar absorption test.
Serum levels of total bilirubin and bile acid were found to be lower in the PN + Bif group, with considerably improved ileum and liver histology (vs. the PN group). The bacterial translocation rate (15.6%), serum endotoxin level (0.11 ± 0.03 EU/ml), and lactulose/mannitol ratio (0.02 ± 0.004) in the PN + Bif group were obviously lower than those of PN group (77.5%, 0.60 ± 0.09 EU/ml, and 0.038 ± 0.008, respectively) and similar to those of the control group (2.8%, 0.09 ± 0.03 EU/ml, and 0.019 ± 0.005, respectively).
Enteral probiotic supplementation could reduce gut permeability, bacterial translocation and endotoxemia, and thus attenuate PN-associated gut and liver injuries in infant rabbits.
肠外营养（PN）导致的肝损伤与肠道萎缩有关，益生菌已被证明具有稳定肠道微生态系统的能力，并能防止肠道细菌易位到肝脏。因此，我们假设肠内双歧杆菌补充剂可以减轻 PN 相关的肝损伤。
将 3 周龄新西兰兔分为三组：对照组、PN 组和 PN+Bif 组（PN 加用双歧杆菌 0.5×10（8）个/天）。10 天后，检测血清肝酶和内毒素水平，并进行肝和回肠组织学检查。肠系膜淋巴结、肺和脾的血液和匀浆样本进行细菌移位培养。通过糖吸收试验测定肠道通透性。
PN+Bif 组血清总胆红素和胆汁酸水平较低，回肠和肝组织学明显改善（与 PN 组相比）。PN+Bif 组的细菌移位率（15.6%）、血清内毒素水平（0.11±0.03EU/ml）和乳果糖/甘露醇比值（0.02±0.004）明显低于 PN 组（77.5%、0.60±0.09EU/ml 和 0.038±0.008），与对照组（2.8%、0.09±0.03EU/ml 和 0.019±0.005）相似。
肠内益生菌补充可降低肠道通透性、细菌易位和内毒素血症，从而减轻婴儿兔 PN 相关的肠道和肝脏损伤。
