使用SPOT技术合成抗菌肽。

Synthesis of antimicrobial peptides using the SPOT technique.

作者信息

Winkler Dirk F H, Hilpert Kai

机构信息

Brain Research Centre, University of British Columbia, Vancouver, BC, Canada.

出版信息

Methods Mol Biol. 2010;618:111-24. doi: 10.1007/978-1-60761-594-1_8.

DOI:10.1007/978-1-60761-594-1_8

PMID:20094861

Abstract

Developing new lead structures for drugs against multiresistant bacteria is an urgent need for modern medicine. Antimicrobial peptides are a class of drugs that can be used to discover such structures. In order to support development of this research, a fast, easy, and inexpensive method to synthesize peptides is necessary. The SPOT synthesis has the potential to produce the required peptide arrays, synthesizing up to 8,000 peptides, peptide mixtures, or other organic compounds on cellulose or other planar surfaces in a positionally addressable and multiple manner. Protocols for the preparation of cellulose membranes and the SPOT synthesis as well as cleavage of peptides from the support are described.

摘要

开发针对多重耐药细菌的新型药物先导结构是现代医学的迫切需求。抗菌肽是一类可用于发现此类结构的药物。为了支持这项研究的开展，需要一种快速、简便且廉价的肽合成方法。SPOT合成法有潜力生成所需的肽阵列，能够在纤维素或其他平面表面上以位置可寻址且多样的方式合成多达8000种肽、肽混合物或其他有机化合物。本文描述了纤维素膜的制备、SPOT合成以及从载体上切割肽的实验方案。