Matsumoto S, Hanai T, Shimizu N, Sugimoto K, Uemura H
Department of Urology, Kinki University School of Medicine, Japan.
Aktuelle Urol. 2010 Jan;41 Suppl 1:S46-9. doi: 10.1055/s-0029-1224656. Epub 2010 Jan 21.
Edaravone is a newly developed radical scavenging agent that has been widely used for protection against ischemia/reperfusion (I/R) injury in patients with cerebral infarction. The present study investigated the effects of edaravone on the I/R injury in rat urinary bladder. Adult male rats were divided in the four groups: groups 1-3 received 1 hour of ischemia followed by 1 hour of reperfusion with saline and with edaravone (1 and 3 mg/kg body weight), and group 4 were age-matched control rats. The in vivo ischemia was created by clamping the vesical arteries for 1 hour and reperfusion was accomplished by removing the clips and lasted for 1 hour. Edaravone or saline were administered after reperfusion for 30 min. Following reperfusion, the bladder was excised and separated. Bladder smooth muscle cell (SMC) phenotypic expression was investigated in the electron microscope. The number of contractile and non-contractile bladder SMC phenotype according to the morphological criteria was counted and the ratio of non-contractile to contractile phenotype was calculated. The responses to electrical field stimulation and carbachol were recorded. Edaravone administration resulted in the protection of the morphological changes and contractile responses to both EFS and carbachol that were affected by the agent. Our findings demonstrate that edaravone has a potentially protective effect on I/R-induced damage in the rat bladder.
依达拉奉是一种新开发的自由基清除剂，已被广泛用于保护脑梗死患者免受缺血/再灌注（I/R）损伤。本研究调查了依达拉奉对大鼠膀胱I/R损伤的影响。成年雄性大鼠分为四组：第1-3组经历1小时缺血，随后分别用生理盐水和依达拉奉（1和3mg/kg体重）进行1小时再灌注，第4组为年龄匹配的对照大鼠。通过夹闭膀胱动脉1小时造成体内缺血，移除夹子实现再灌注，持续1小时。再灌注30分钟后给予依达拉奉或生理盐水。再灌注后，切除并分离膀胱。在电子显微镜下研究膀胱平滑肌细胞（SMC）表型表达。根据形态学标准计数收缩性和非收缩性膀胱SMC表型的数量，并计算非收缩性与收缩性表型的比例。记录对电场刺激和卡巴胆碱的反应。给予依达拉奉可保护受该药物影响的形态学变化以及对电场刺激和卡巴胆碱的收缩反应。我们的研究结果表明，依达拉奉对大鼠膀胱I/R诱导的损伤具有潜在的保护作用。
