二甲双胍对去卵巢Sprague-Dawley大鼠血清性激素、瘦素和胰岛素水平的影响。

Effects of metformin on serum levels of sex hormone, leptin and insulin in ovariectomized Sprague-Dawley rats.

作者信息

Yan Li, Kang Bai, Li Guangzhou, Yin Zhaozhen, Wang Yanyan

机构信息

Department of Pharmacology, Weifang Medical College, Weifang, P R China.

出版信息

Pharmazie. 2009 Dec;64(12):834-5.

PMID:20095143

Abstract

The aim of the present experiment was to study the effects of metformin (MF) on serum concentrations of sex hormone, leptin and insulin in ovariectomized (OVX) Sprague-Dawley (SD) rats. The serum estradiol (E2), progesterone (P), leptin and insulin concentrations were measured with radioimmunoassay (RIA). Compared with SHAM operated rats, the serum concentrations of E2 and P were significantly lower (P < 0.01), but leptin and insulin were significantly higher in OVX rats (P < 0.01). MF (135mg kg(-1) d(-1), ig, 30 d) had no evident effects on serum concentrations of E2, P, leptin and insulin in OVX rats. MF (270 mg kg(-1) d(-1), ig, 30 d) markedly increased serum concentrations of E2 and P (P < 0.05) while decreased serum concentrations of leptin and insulin (P < 0.05 and P < 0.01) in OVX rats. We concluded that long-term treatment of high dose MF cannot only significantly decrease serum concentrations of leptin and insulin, but also increase E2 and P in OVX SD rats.

摘要

本实验旨在研究二甲双胍（MF）对去卵巢（OVX）的Sprague-Dawley（SD）大鼠血清性激素、瘦素和胰岛素浓度的影响。采用放射免疫分析法（RIA）测定血清雌二醇（E2）、孕酮（P）、瘦素和胰岛素浓度。与假手术大鼠相比，OVX大鼠血清E2和P浓度显著降低（P < 0.01），但瘦素和胰岛素显著升高（P < 0.01）。MF（135mg kg(-1) d(-1)，灌胃，30天）对OVX大鼠血清E2、P、瘦素和胰岛素浓度无明显影响。MF（270 mg kg(-1) d(-1)，灌胃，30天）可显著提高OVX大鼠血清E2和P浓度（P < 0.05），同时降低血清瘦素和胰岛素浓度（P < 0.05和P < 0.01）。我们得出结论，长期高剂量MF治疗不仅能显著降低OVX SD大鼠血清瘦素和胰岛素浓度，还能提高E2和P浓度。