Ifremer, Laboratoire de Génétique et Pathologie, 17390, La Tremblade, France.
Anim Genet. 2010 Aug;41(4):390-9. doi: 10.1111/j.1365-2052.2009.02018.x. Epub 2010 Jan 21.
Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F(2) full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of single full-sib families revealed differential QTL segregation between families. QTL for the two-recorded traits presented very similar locations, highlighting the interest of further study of their respective genetic controls. These QTL show substantial genetic variation in resistance to summer mortality, and present new opportunities for selection for resistance to OsHV-1.
夏季死亡是严重影响太平洋牡蛎（Crassostrea gigas）水产养殖生产的现象。尽管其致病因素复杂，但对死亡率的抗性已被描述为一种高度可遗传的特征，并且包括病毒牡蛎疱疹病毒 1 型（OsHV-1）在内的几种病原体与该现象有关。使用实时 PCR 对 5 个 F2 全同胞家系进行了夏季死亡率和 OsHV-1 负荷的生存 QTL 分析，这些家系是为了对夏季死亡率的抗性而进行的分歧选择实验的结果。使用 29 个 SNP 和 51 个微卫星标记构建了共识连锁图谱。鉴定并分配了五个显著的 QTL 到连锁群 V、VI、VII 和 IX。对单个全同胞家系的分析表明，家系之间存在 QTL 分离的差异。记录的两个性状的 QTL 位置非常相似，突出了进一步研究其各自遗传控制的重要性。这些 QTL 显示了对夏季死亡率的抗性具有显著的遗传变异，为对 OsHV-1 的抗性选择提供了新的机会。
