Department of Clinical Parasitology, Hospital for Tropical Diseases, 3rd Floor, Mortimer Market, Capper Street, London WC1E 6JB, UK.
Trans R Soc Trop Med Hyg. 2010 Apr;104(4):255-8. doi: 10.1016/j.trstmh.2009.12.004. Epub 2010 Jan 22.
We have developed a new ELISA for detection of anti-schistosome antibodies using an extract from Schistosoma mansoni cercariae. We evaluated the new assay on serum samples sent to the Hospital for Tropical Diseases, Department of Clinical Parasitology, London, UK, by comparing it with our routinely used S. mansoni soluble egg antigen (SEA) assay. We also evaluated the new assay for cross-reactivity with a number of helminth and other infections. We demonstrate that the cercarial antigen assay is equivalent to the SEA assay for serodiagnosis of schistosomiasis in a non-endemic setting. The cercarial antigen preparation is more easily produced than SEA, and for this reason this assay may be preferred for routine clinical use and may be amenable to scaling up. Further assessment is needed before it can be recommended for use in an endemic area, as chronic disease and co-infection with other helminths are likely to be under-represented in our sample set.
我们使用曼氏血吸虫尾蚴提取物开发了一种新的 ELISA 来检测抗血吸虫抗体。我们通过将新检测方法与我们常规使用的曼氏血吸虫可溶性虫卵抗原（SEA）检测方法进行比较，对来自英国伦敦热带病医院临床寄生虫学系的血清样本进行了评估。我们还评估了新检测方法对多种寄生虫和其他感染的交叉反应性。我们证明，在非流行地区，尾蚴抗原检测方法与 SEA 检测方法在血吸虫病血清诊断方面等效。与 SEA 相比，尾蚴抗原制剂更容易制备，因此该检测方法可能更适合常规临床使用，并且可能易于扩大规模。在推荐在流行地区使用之前，还需要进一步评估，因为在我们的样本集中可能无法充分代表慢性疾病和与其他寄生虫的合并感染。
