普通欧螈南方亚种（两栖纲，有尾目）的两个分散的高度重复DNA家族在蝾螈科中广泛保守。

Two dispersed highly repeated DNA families of Triturus vulgaris meridionalis (Amphibia, Urodela) are widely conserved among Salamandridae.

作者信息

Vignali R, Rijli F M, Batistoni R, Fratta D, Cremisi F, Barsacchi G

机构信息

Dipartimento di Fisiologia e Biochimica, Laboratori di Biologia Cellulare e dello Sviluppo, Pisa, Italy.

出版信息

Chromosoma. 1991 Feb;100(2):87-96. doi: 10.1007/BF00418241.

DOI:10.1007/BF00418241

PMID:2009819

Abstract

Two BamHI families of repeated sequences were characterized from the genome of the Italian smooth newt, Triturus vulgaris meridionalis (Amphibia, Urodela). The first family, which is divided into subfamilies, consists of tandemly arranged arrays whose basic repeat is around 398 bp long; these arrays are dispersed throughout the entire chromosome sets of the various species of Triturus tested. Moreover the family is widely conserved among Salamandridae, being detected by genomic DNA blotting of Notophthalmus viridescens, Taricha granulosa, Salamandrina terdigitata and Euproctus platycephalus. The second BamHI family is represented by a cloned sequence of 419 bp, which is dispersed in the chromosome set of several species of Triturus. The sequence is also conserved in S. terdigitata and in E. platycephalus but is not detectable in N. viridescens or T. granulosa. The cloned sequence is most probably only part of a longer unit interspersed within the Triturus genome.

摘要

从意大利光滑蝾螈（Triturus vulgaris meridionalis，两栖纲，有尾目）的基因组中鉴定出了两个BamHI重复序列家族。第一个家族可分为亚家族，由串联排列的阵列组成，其基本重复片段长度约为398 bp；这些阵列分散在受试的各种Triturus物种的整个染色体组中。此外，该家族在蝾螈科中广泛保守，通过对绿红东美螈（Notophthalmus viridescens）、粗皮渍螈（Taricha granulosa）、指趾蝾螈（Salamandrina terdigitata）和平头欧螈（Euproctus platycephalus）的基因组DNA印迹分析得以检测到。第二个BamHI家族由一个419 bp的克隆序列代表，该序列分散在几种Triturus物种的染色体组中。该序列在指趾蝾螈和平头欧螈中也保守，但在绿红东美螈或粗皮渍螈中未检测到。该克隆序列很可能只是散布在Triturus基因组中的一个更长单元的一部分。