Da Silva Maria C, De Lemos Raimunda N S, Lima Luzia H C, Gourlart Filho Luiz R, Pereira Silma R F
Programa de Pós-Graduação em Agroecologia, UEMA, C. postal 9, 65.055-098, São Luís, MA.
Neotrop Entomol. 2009 Nov-Dec;38(6):762-8. doi: 10.1590/s1519-566x2009000600009.
The RAPD technique is widely used to investigate the distinct genetic characteristics of the complex Bemisia tabaci (Gennadius), which is currently constituted of approximately 41 biotypes. The objective of this research was to characterize populations of whitefly collected in crops of agricultural producing areas in São Luís, MA, like okra, beans and pepper, using RAPD molecular markers. Females from nine whitefly populations were analyzed and compared with B. tabaci biotype B taken from poinsettia culture of Embrapa Genetic Resources and Biotechnology (Brasília, DF). Twelve out of the 20 primers tested produced specific band patterns suitable to confirm that the evaluated specimens belong to the biotype B of B. tabaci, despite the high percentage of detected polymorphism. The analysis of the 96 RAPD molecular markers generated indicated that the populations on okra, beans and pepper were grouped according to the host cultures, sharing 80, 76 and 45% of genetic similarity, respectively, when compared with the control population of B. tabaci biotype B. A lower selective pressure was observed with the population of whitefly collected on pepper and minor genetic variability in the whitefly populations collected on okra and bean, when compared with the control population.
随机扩增多态性DNA（RAPD）技术被广泛用于研究复杂的烟粉虱（Gennadius）的独特遗传特征，目前烟粉虱大约由41个生物型组成。本研究的目的是使用RAPD分子标记对在马塞约州圣路易斯农业产区的作物（如秋葵、豆类和辣椒）上采集的粉虱种群进行特征分析。分析了来自九个粉虱种群的雌性个体，并与从巴西农业研究公司遗传资源与生物技术中心（巴西利亚，联邦区）的一品红培养物中获取的烟粉虱生物型B进行比较。在所测试的20种引物中，有12种产生了适合确认所评估样本属于烟粉虱生物型B的特异性条带模式，尽管检测到的多态性比例很高。对所产生的96个RAPD分子标记的分析表明，秋葵、豆类和辣椒上的粉虱种群根据寄主作物进行分组，与烟粉虱生物型B的对照种群相比，它们分别共享80%、76%和45%的遗传相似性。与对照种群相比，在辣椒上采集的粉虱种群观察到较低的选择压力，而在秋葵和豆类上采集的粉虱种群遗传变异性较小。
