Ley V, Langlade-Demoyen P, Kourilsky P, Larsson-Sciard E L
Unité de Biologie Moléculaire du Gène, U.277 INSERM, U.A.C. 535 CNRS, Institut Pasteur, Paris, France.
Eur J Immunol. 1991 Mar;21(3):851-4. doi: 10.1002/eji.1830210350.
We have quantitatively studied the effect of interleukin (IL) 2 on the cytotoxic T lymphocyte (CTL) response to tumor cells in vivo. Mastocytoma P815 was transfected with murine IL 2 cDNA (P815-IL 2) and injected into syngeneic mice. The anti-tumor response was analyzed and compared with the response induced by the non-transfected cells. P815 parental cells are highly tumorigenic, causing death within 20-30 days. In contrast, IL 2-transfected cells were totally rejected. Co-injection of IL 2-secreting and parental cells resulted in the inhibition of growth of both type of tumors. In addition, the response induced by IL 2-secreting cells protected the mice against a subsequent challenge with P815. Long-term memory persisted in treated mice 3 months after tumor rejection. Frequencies of CTL precursors and CTL specific for P815 increased as a result of IL 2 secretion by the target cells. Estimates of CTL frequency at days 8 and 12 after injection were 2 to 3 times higher in mice inoculated with P815-IL 2 cells, and this correlated with tumor rejection.
我们已经定量研究了白细胞介素（IL）-2对体内细胞毒性T淋巴细胞（CTL）针对肿瘤细胞的反应的影响。用小鼠IL-2 cDNA转染肥大细胞瘤P815（P815-IL-2），并将其注射到同基因小鼠体内。分析抗肿瘤反应，并与未转染细胞诱导的反应进行比较。P815亲本细胞具有高度致瘤性，可在20-30天内导致死亡。相比之下，IL-2转染细胞被完全排斥。共同注射分泌IL-2的细胞和亲本细胞导致两种类型肿瘤的生长均受到抑制。此外，分泌IL-2的细胞诱导的反应使小鼠免受随后P815的攻击。肿瘤排斥3个月后，治疗小鼠中持续存在长期记忆。由于靶细胞分泌IL-2，CTL前体和针对P815的CTL的频率增加。注射后第8天和第12天，接种P815-IL-2细胞的小鼠中CTL频率估计高出2至3倍，这与肿瘤排斥相关。
