氯霉素乙酰转移酶基因通过高速机械法导入器官外植体及体内的啮齿动物肝脏、肾脏和乳腺细胞。

High-velocity mechanical DNA transfer of the chloramphenicolacetyl transferase gene into rodent liver, kidney and mammary gland cells in organ explants and in vivo.

作者信息

Zelenin A V, Alimov A A, Titomirov A V, Kazansky A V, Gorodetsky S I, Kolesnikov V A

机构信息

Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1991 Mar 11;280(1):94-6. doi: 10.1016/0014-5793(91)80212-l.

DOI:10.1016/0014-5793(91)80212-l

PMID:2009972

Abstract

Mouse and rat liver, kidney and mammary gland explants were bombarded with high-velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT-cat, p chi-Casein-cat, p beta-Casein-cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT-CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying pTAT-cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.

摘要

用携带在不同启动子（pTAT-cat、p chi-酪蛋白-cat、pβ-酪蛋白-cat）控制下的氯霉素乙酰转移酶基因的高速微弹轰击小鼠和大鼠的肝脏、肾脏及乳腺外植体。转染24小时后，在所有器官外植体中均检测到CAT基因的表达。当使用TAT-CAT构建体时，表达最为明显。在体内实验中，用携带pTAT-cat DNA的微弹原位轰击大鼠肝脏。轰击24小时后检测到CAT基因有显著活性。