Laboratory for Infectious Diseases and Screening (LIS) Centre for Infectious Disease Control, National Institute for Public Health and the Environment - RIVM - Netherlands, 3720 BA Bilthoven, The Netherlands.
BMC Genomics. 2010 Jan 26;11:64. doi: 10.1186/1471-2164-11-64.
Bordetella pertussis is the causative agent of human whooping cough (pertussis) and is particularly severe in infants. Despite worldwide vaccinations, whooping cough remains a public health problem. A significant increase in the incidence of whooping cough has been observed in many countries since the 1990s. Several reasons for the re-emergence of this highly contagious disease have been suggested. A particularly intriguing possibility is based on evidence indicating that pathogen adaptation may play a role in this process. In an attempt to gain insight into the genomic make-up of B. pertussis over the last 60 years, we used an oligonucleotide DNA microarray to compare the genomic contents of a collection of 171 strains of B. pertussis isolates from different countries.
The CGH microarray analysis estimated the core genome of B. pertussis, to consist of 3,281 CDSs that are conserved among all B. pertussis strains, and represent 84.8% of all CDSs found in the 171 B. pertussis strains. A total of 64 regions of difference consisting of one or more contiguous CDSs were identified among the variable genes. CGH data also revealed that the genome size of B. pertussis strains is decreasing progressively over the past 60 years. Phylogenetic analysis of microarray data generated a minimum spanning tree that depicted the phylogenetic structure of the strains. B. pertussis strains with the same gene content were found in several different countries. However, geographic specificity of the B. pertussis strains was not observed. The gene content was determined to highly correlate with the ptxP-type of the strains.
An overview of genomic contents of a large collection of isolates from different countries allowed us to derive a core genome and a phylogenetic structure of B. pertussis. Our results show that B. pertussis is a dynamic organism that continues to evolve.
百日咳博德特氏菌是人类百日咳（百日咳）的病原体，尤其在婴儿中更为严重。尽管全世界都进行了疫苗接种，但百日咳仍是一个公共卫生问题。自 20 世纪 90 年代以来，许多国家观察到百日咳的发病率显著上升。这种高度传染性疾病再次出现的原因有几个。一个特别有趣的可能性是基于表明病原体适应可能在这个过程中起作用的证据。为了深入了解过去 60 年来 B. pertussis 的基因组构成，我们使用寡核苷酸 DNA 微阵列比较了来自不同国家的 171 株 B. pertussis 分离株的基因组含量。
CGH 微阵列分析估计 B. pertussis 的核心基因组由 3281 个 CDS 组成，这些 CDS 在所有 B. pertussis 菌株中保守，占 171 株 B. pertussis 菌株中所有 CDS 的 84.8%。在可变基因中鉴定出由一个或多个连续 CDS 组成的 64 个差异区。CGH 数据还表明，B. pertussis 菌株的基因组大小在过去 60 年中逐渐减小。微阵列数据的系统发育分析生成了一个最小生成树，描绘了菌株的系统发育结构。在几个不同的国家发现了具有相同基因含量的 B. pertussis 菌株。然而，没有观察到 B. pertussis 菌株的地理特异性。基因含量与菌株的 ptxP 型高度相关。
对来自不同国家的大量分离株的基因组含量进行概述，使我们能够得出 B. pertussis 的核心基因组和系统发育结构。我们的结果表明，B. pertussis 是一种不断进化的动态生物体。
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