State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
Clin Oral Implants Res. 2010 Apr 1;21(4):392-7. doi: 10.1111/j.1600-0501.2009.01857.x. Epub 2010 Jan 22.
The biological properties of nano-hydroxyapatite/polyamide (nHA/PA66) membrane as a guided tissue regeneration barrier were investigated based on a critical-size calvarial defect model.
Thirty-six Sprague-Dawley rats were divided into four groups: nHA/PA66 membranes, control treated with expanded polytetrafluoroethylene (e-PTFE) membranes, control treated with PA membranes and blank. Bone regeneration in each group was evaluated using morphological, serological, radiographic and histological techniques at 1, 4 and 8 weeks, respectively, post-operation.
Slight degradation of nHA/PA66 membranes was observed by a scanning electron microscope (SEM) at 4 and 8 weeks. The serum alkaline phosphatase level and the integral optical density of neo-bone were significantly higher in the nHA/PA66 group than those in the PA group and blank (P<0.05). Radiographic and histological analysis showed a fully closed cranial defect for both the nHA/PA66 group and the e-PTFE group at 8 weeks. No remarkable difference was found between the nHA/PA66 group and the e-PTFE group at each time interval.
nHA/PA66 membrane promoted bone regeneration as effectively as conventional e-PTFE membrane. Based on a rat study, nHA/PA66 membrane provides a promising alternative to e-PTFE membrane due to the absence of requirement for a second surgery.
基于临界尺寸颅骨缺损模型，研究纳米羟基磷灰石/聚酰胺（nHA/PA66）膜作为引导组织再生屏障的生物学特性。
36 只 Sprague-Dawley 大鼠随机分为 4 组：nHA/PA66 膜组、对照组 e-PTFE 膜组、对照组 PA 膜组和空白组。术后 1、4 和 8 周，分别采用形态学、血清学、影像学和组织学技术评估各组骨再生情况。
扫描电镜（SEM）观察到 nHA/PA66 膜在 4 周和 8 周时有轻微降解。nHA/PA66 组血清碱性磷酸酶水平和新骨积分光密度均明显高于 PA 组和空白组（P<0.05）。影像学和组织学分析显示，nHA/PA66 组和 e-PTFE 组在 8 周时颅骨缺损均完全闭合。nHA/PA66 组和 e-PTFE 组在每个时间点均无显著差异。
nHA/PA66 膜可有效促进骨再生，与传统 e-PTFE 膜效果相当。基于大鼠研究，nHA/PA66 膜为 e-PTFE 膜提供了一种有前途的替代方案，因为它不需要进行第二次手术。
