State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Int J Mol Sci. 2009 Jul 24;10(8):3269-3282. doi: 10.3390/ijms10083269.
Phytochelatins (PCs) play an important role in detoxification of heavy metals in plants. PCs are synthesized from glutathione by phytochelatin synthase (PCS), a dipeptidyltransferase. Sesbania rostrata is a tropical legume plant that can tolerate high concentrations of Cd and Zn. In this study, the S. rostrata PCS gene (SrPCS) and cDNAs were isolated and characterized. Southern blot and sequence analysis revealed that a single copy of the SrPCS gene occurs in the S. rostrata genome, and produces four different SrPCS mRNAs and proteins, SrPCS1-SrPCS4, by alternative splicing of the SrPCS pre-mRNA. The SrPCS1 and SrPCS3 proteins conferred Cd tolerance when expressed in yeast cells, whereas the SrPCS2 and SrPCS4 proteins, which lack the catalytic triad and the N-terminal domains, did not. These results suggested that SrPCS1 and SrPCS3 have potential applications in genetic engineering of plants for enhancing heavy metal tolerance and phytoremediation of contaminated soils.
植物螯合肽（PCs）在植物解毒重金属中起着重要作用。PCs 是由植物螯合肽合酶（PCS）从谷胱甘肽合成的，PCS 是一种二肽转移酶。田菁是一种热带豆科植物，能够耐受高浓度的 Cd 和 Zn。在这项研究中，分离并鉴定了田菁 PCS 基因（SrPCS）及其 cDNA。Southern 印迹和序列分析表明，SrPCS 基因在田菁基因组中只有一个拷贝，通过 SrPCS 前体 mRNA 的选择性剪接产生四种不同的 SrPCS mRNAs 和蛋白质，SrPCS1-SrPCS4。SrPCS1 和 SrPCS3 蛋白在酵母细胞中表达时赋予 Cd 耐受性，而缺乏催化三联体和 N 端结构域的 SrPCS2 和 SrPCS4 蛋白则没有。这些结果表明，SrPCS1 和 SrPCS3 有可能应用于植物的遗传工程，以增强对重金属的耐受性和对污染土壤的植物修复。
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