Huang Ke, Zhang Li, Liu Zhuxiang, Chen Qihui, Peng Qingzhong, Li Wenjun, Cui Xiaolong, Chen Yiguang
Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China.
Wei Sheng Wu Xue Bao. 2009 Nov;49(11):1424-9.
To investigate the diversity of culturable bacteria isolated from the sea urchin Hemicentrotus pulcherrimus collected from a tidal flat of Naozhou Island (20 degrees 52'N - 20 degrees 56'N 110 degrees 33'E - 110 degrees 38'E), Leizhou Bay, South China Sea, China.
Bacteria were isolated from the sample by using conventional culture-dependent method and then investigated by using phylogenetic analysis based on 16S rRNA gene sequence comparisons.
We isolated 106 bacterial strains from the sample on media (Difco marine 2216, International Streotomyces Project medium 2, nutrient, sea water and humic acid agars) supplemented with 0 - 2 mol/L NaCl. On the basis of morphological, physiological and biochemical characteristics, we selected 34 strains to perform a phylogenetic analysis based on 16S rRNA gene sequences. Our results showed that 34 isolates represented 21 species, belonging to 17 genera (Alteromonas, Bacillus, Brachybacterium, Brevibacterium, Halobacillus, Halomonas, Nocardiopsis, Oceanobacillus, Piscibacillus, Planococcus, Pontibacillus, Pseudoalteromonas, Pseudonocardia, Salinicoccus, Salinivibrio, Staphylococcus, Vibrio, Virgibacillus) of 10 families (Alteromonadaceae, Bacillaceae, Brevibacteriaceae, Dermabacteraceae, Halomonadaceae, Planococcaceae, Pseudoalteromonadaceae, Pseudonocardiaceae, Nocardiopsaceae, Staphylococcaceae, Vibrionaceae) in three phylogenetic groups (Actinobacteria, Firmicutes, Gamma-Proteobacteria). The most abundant and diverse isolates were within the phylum Firmicutes (58.8%) and the subphylum Gamma-Proteobacteria (26.5%). The phylogenetic distance matrix results suggested that there were obvious genetic divergences between most isolates and their closestly related type strains (16S rRNA gene sequence similarities ranged from 99.6 to 99.9%), and that, out of 34 isolates, at least 5 strains (JSM 076033, JSM 076056, JSM 076093, JSM 078063, JSM 078169) could represent 5 potential new species within 5 characterized genera (Jeotgalicoccus, Pontibacillus, Halomonas, Bacillus, Sporosarcina), in which the descriptions of 3 novel species [Jeotgalicoccus marinus (type strain, JSM 076033), Pontibacillus halophilus (JSM 076056), Halomonas zhanjiangensis (JSM 078169)] have been published in IJSEM (International Journal of Systematic and Evolutionary Microbiology).
The results presented above showed that there are abundant bacteria diversity in the sea urchin Hemicentrotus pulcherrimus collected from Naozhou Island.
研究从中国南海雷州湾硇洲岛(北纬20°52′ - 20°56′,东经110°33′ - 110°38′)潮间带采集的光棘球海胆中可培养细菌的多样性。
采用传统的依赖培养方法从样本中分离细菌,然后基于16S rRNA基因序列比较进行系统发育分析。
我们在添加0 - 2 mol/L氯化钠的培养基(Difco海洋2216、国际链霉菌项目培养基2、营养琼脂、海水琼脂和腐殖酸琼脂)上从样本中分离出106株细菌菌株。根据形态、生理和生化特征,我们选择34株菌株基于16S rRNA基因序列进行系统发育分析。结果表明,34株分离株代表21个物种,属于10个科(交替单胞菌科、芽孢杆菌科、短杆菌科、皮肤杆菌科、嗜盐单胞菌科、动性球菌科、假交替单胞菌科、假诺卡氏菌科、诺卡氏放线菌科、葡萄球菌科、弧菌科)的17个属(交替单胞菌属、芽孢杆菌属、短杆菌属、短棒杆菌属、嗜盐芽孢杆菌属、嗜盐单胞菌属、诺卡氏放线菌属、海洋芽孢杆菌属、鱼芽孢杆菌属、动性球菌属、盐土芽孢杆菌属、假交替单胞菌属、假诺卡氏菌属、盐球菌属、盐弧菌属、葡萄球菌属、弧菌属、维氏芽孢杆菌属),分属于三个系统发育类群(放线菌门、厚壁菌门、γ-变形菌纲)。最丰富和多样的分离株存在于厚壁菌门(58.8%)和γ-变形菌纲亚门(26.5%)中。系统发育距离矩阵结果表明,大多数分离株与其最密切相关的模式菌株之间存在明显的遗传差异(16S rRNA基因序列相似性范围为99.6%至99.9%),并且在34株分离株中,至少有5株(JSM 076033、JSM 076056、JSM 076093、JSM 078063、JSM 078169)可能代表5个已鉴定属(嗜盐栖热放线菌属、盐土芽孢杆菌属、嗜盐单胞菌属、芽孢杆菌属、孢芽孢八叠球菌属)内的5个潜在新物种,其中3个新物种[海洋嗜盐栖热放线菌(模式菌株,JSM 076033)、嗜盐盐土芽孢杆菌(JSM 076056)、湛江嗜盐单胞菌(JSM 078169)]已发表于《国际系统与进化微生物学杂志》(IJSEM)。
上述结果表明,从硇洲岛采集的光棘球海胆中存在丰富的细菌多样性。