Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Insect Mol Biol. 2010 Jun 1;19(3):303-13. doi: 10.1111/j.1365-2583.2009.00984.x. Epub 2009 Dec 23.
Bowman-Birk inhibitor (BBI) is toxic when fed to certain insects, including the fruit fly, Drosophila melanogaster. Dietary BBI has been demonstrated to slow growth and increase insect mortality by inhibiting the digestive enzymes trypsin and chymotrypsin, resulting in a reduced supply of amino acids. In mammals, BBI influences cellular energy metabolism. Therefore, we tested the hypothesis that dietary BBI affects energy-associated pathways in the D. melanogaster midgut. Through microarray and metabolomic analyses, we show that dietary BBI affects energy utilization pathways in the midgut cells of D. melanogaster. In addition, ultrastructure studies indicate that microvilli are significantly shortened in BBI-fed larvae. These data provide further insights into the complex cellular response of insects to dietary protease inhibitors.
鲍曼-比克抑制剂（BBI）对某些昆虫有毒，包括果蝇，黑腹果蝇。饮食中的 BBI 已被证明通过抑制消化酶胰蛋白酶和糜蛋白酶来减缓生长并增加昆虫死亡率，从而导致氨基酸供应减少。在哺乳动物中，BBI 影响细胞能量代谢。因此，我们测试了这样一个假设，即饮食中的 BBI 会影响黑腹果蝇中肠的与能量相关的途径。通过微阵列和代谢组学分析，我们表明饮食中的 BBI 会影响黑腹果蝇中肠细胞的能量利用途径。此外，超微结构研究表明，BBI 喂养的幼虫的微绒毛明显缩短。这些数据为昆虫对饮食蛋白酶抑制剂的复杂细胞反应提供了进一步的见解。
