血栓素合酶被氢过氧脂肪酸灭活。
Inactivation of thromboxane synthase with hydroperoxy-fatty acids.
作者信息
Hall E R, Townsend G, Frasier-Scott K F
机构信息
Department of Internal Medicine, University of Texas Medical School, Houston.
出版信息
Prostaglandins Leukot Essent Fatty Acids. 1991 Jan;42(1):19-22. doi: 10.1016/0952-3278(91)90061-9.
Bovine lung thromboxane synthase was immobilized on phenyl-Sepharose beads by adsorption. The immobilized enzyme was catalytically active and synthesized both TXA2 and HHT. The structure-activity relationship of several hydroperoxy fatty acids and their ability to inactivate thromboxane synthase was investigated. Millimolar quantities of hydrogen peroxide and tert-butylperoxide were required to inactivate the enzyme: whereas micromolar quantities of C18 and C20 hydroperoxy fatty acids inactivated the enzyme. Pretreatment of the enzyme with long chain hydroperoxy-fatty acids resulted in a decreased synthesis of both TXB2 and HHT.
牛肺血栓素合酶通过吸附作用固定在苯基琼脂糖珠上。固定化酶具有催化活性，能合成血栓素A2（TXA2）和12-L-羟基-5,8,10,14-二十碳四烯酸（HHT）。研究了几种氢过氧脂肪酸的构效关系及其使血栓素合酶失活的能力。使该酶失活需要毫摩尔量的过氧化氢和叔丁基过氧化物；而微摩尔量的C18和C20氢过氧脂肪酸就能使该酶失活。用长链氢过氧脂肪酸对该酶进行预处理会导致TXB2和HHT的合成减少。
Zotero 插件