University of Wisconsin-Madison, Laboratory of Molecular Biology, 1525 Linden Drive, Madison, WI 53706, USA.
Curr Biol. 2010 Feb 9;20(3):259-64. doi: 10.1016/j.cub.2009.12.045. Epub 2010 Jan 28.
Cell division requires coordinated regulation of chromosome segregation and cytokinesis. Although much is known about the function of the protease separase in promoting sister chromosome separation, the role of separase during cytokinesis is unclear. We show that separase localizes to the ingressing furrow and midbody during cytokinesis in the C. elegans embryo. Loss of separase function during the early mitotic divisions causes cytokinesis failure that is not due to eggshell defects or chromosome nondisjunction. Moreover, depletion of separase causes the accumulation of RAB-11-positive vesicles at the cleavage furrow and midbody that is not a consequence of chromosome nondisjunction, but is mimicked by depletion of vesicle fusion machinery. Collectively, these data indicate that separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody.
细胞分裂需要协调调节染色体分离和胞质分裂。虽然蛋白酶分离酶在促进姐妹染色体分离方面的功能已经得到了广泛的了解，但它在胞质分裂中的作用尚不清楚。我们发现，在 C. elegans 胚胎的胞质分裂过程中，分离酶定位于入核沟和中体。在早期有丝分裂过程中分离酶功能丧失会导致胞质分裂失败，但这不是由于卵壳缺陷或染色体不分离引起的。此外，分离酶的耗竭会导致 RAB-11 阳性囊泡在分裂沟和中体处的积累，这不是染色体不分离的结果，而是通过耗尽囊泡融合机制来模拟的。总的来说，这些数据表明，分离酶通过调节 RAB-11 阳性囊泡在分裂沟和中体处整合到质膜中，从而对胞质分裂是必需的。
