Division of Animal Science, S132 ASRC, University of Missouri, Columbia 65211, USA.
J Anim Sci. 2010 Jun;88(6):2045-54. doi: 10.2527/jas.2009-2627. Epub 2010 Jan 29.
Two experiments evaluated short-term controlled internal drug release (CIDR) insert-based protocols to synchronize estrus and ovulation and compare differences in their potential to facilitate fixed-time AI (FTAI) in postpartum beef cows. Experiment 1 was designed to compare the 7- and 5-d Select Synch + CIDR protocols on the basis of timing and synchrony of estrus after treatment. Cows assigned to the 7-d protocol (n = 59) received GnRH [100 microg intramuscularly (i.m.)] and CIDR inserts (1.38 g of progesterone) on d 0 and PGF(2alpha) (25 mg i.m.) and CIDR removal on d 7. Cows assigned to the 5-d protocol (n = 58) received GnRH and CIDR inserts on d 2, PGF(2alpha) and CIDR removal on d 7, and a second injection of PGF(2alpha) 12 h after CIDR removal. Estrus detection and AI were performed for cows assigned to each protocol during the 144-h synchronized period. There was no difference in estrous response (P = 0.85), interval to estrus (P = 0.09), or variance for interval to estrus (P = 0.75) between treatments, nor were there differences in synchronized conception or pregnancy rates resulting from AI (P = 0.85, P = 0.91, respectively). Experiment 2 was designed to compare pregnancy rates resulting from FTAI after administration of the 7- and 5-d CO-Synch + CIDR protocols. Both treatments were administered the same as in Exp. 1; however, cows assigned to the 7-d protocol were inseminated 66 h after PGF(2alpha) and CIDR removal, and cows assigned to the 5-d protocol were inseminated 72 h after the first PGF(2alpha) injection. Cows assigned to both protocols were administered GnRH (100 microg i.m.) at AI. There was no effect of treatment (P = 0.85), technician (P = 0.20), or sire (P = 0.25) on pregnancy rates resulting from FTAI. Given these observations, the 5-d protocol provides an effective alternative to the 7-d protocol for use in facilitating FTAI; however, beef producers must carefully consider the increased labor and treatment costs associated with the 5-d protocol.
两个实验评估了基于短期控制内部药物释放(CIDR)插入的方案,以同步发情和排卵,并比较它们在促进产后肉牛定时人工授精(FTAI)方面的潜在差异。实验 1 旨在根据处理后发情的时间和同步性比较 7 天和 5 天 Select Synch + CIDR 方案。将奶牛分为 7 天方案(n = 59),在第 0 天和第 7 天接受 GnRH [100 mcg 肌内(i.m.)]和 CIDR 插入(1.38 g 孕酮)和 PGF(2alpha)(25 mg i.m.)和 CIDR 取出。将奶牛分为 5 天方案(n = 58),在第 2 天接受 GnRH 和 CIDR 插入,在第 7 天接受 PGF(2alpha)和 CIDR 取出,在 CIDR 取出后 12 小时接受第二次 PGF(2alpha)注射。在同步期的 144 小时内,为每个方案分配的奶牛进行发情检测和 AI。发情反应(P = 0.85)、发情间隔(P = 0.09)或发情间隔方差(P = 0.75)在处理之间没有差异,也没有发情 AI 导致的同步受孕或妊娠率差异(P = 0.85,P = 0.91)。实验 2 旨在比较在 7 天和 5 天 CO-Synch + CIDR 方案给药后,通过 FTAI 获得的妊娠率。两种处理方法与实验 1 相同;然而,在第 7 天方案中,在 PGF(2alpha)和 CIDR 取出后 66 小时进行授精,在第 5 天方案中,在第一次 PGF(2alpha)注射后 72 小时进行授精。为两个方案分配的奶牛在 AI 时接受 GnRH(100 mcg i.m.)。FTAI 导致的妊娠率不受处理(P = 0.85)、技术员(P = 0.20)或种公牛(P = 0.25)的影响。鉴于这些观察结果,5 天方案为促进 FTAI 提供了 7 天方案的有效替代方案;然而,肉牛生产者必须仔细考虑与 5 天方案相关的增加劳动力和治疗成本。