Golubev Wladyslav, Sampaio José
Institute for Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
J Gen Appl Microbiol. 2009 Dec;55(6):441-6. doi: 10.2323/jgam.55.441.
Nitrate-positive strains of anamorphic yeasts representing the genus Cryptococcus were isolated on fern infusion agar from Athyrium filix-femina. Phylogenetic analyses of the D1/D2 domain of the LSU rRNA and of the ITS region placed them in Cylindricus clade in the Filobasidiales. Morphological and physiological characteristics, as well as mycocinotyping and molecular analysis, show a close affinity between strains. Subsequent comparative studies of new isolates revealed that they were not conspecific with any other known Cryptococcus species, and the cultures represented an undescribed species with two varieties, for which the names Cryptococcus filicatus var. filicatus (type strain VKM Y-2954 = CBS 10874) and Cryptococcus filicatus var. pelliculosus (type strain VKM Y-2955 = CBS 10875) are proposed.
从欧洲鳞毛蕨中分离出代表隐球菌属的无性型酵母硝酸盐阳性菌株,该菌株是在蕨类浸出液琼脂上分离得到的。对大亚基核糖体RNA的D1/D2结构域和ITS区域进行系统发育分析,将它们置于线黑粉菌目中的圆柱状分支中。形态学、生理学特征以及霉菌素分型和分子分析表明菌株之间具有密切的亲缘关系。随后对新分离株的比较研究表明,它们与任何其他已知的隐球菌物种都不同种,这些培养物代表了一个未描述的物种,有两个变种,为此提出了隐球菌丝状变种丝状隐球菌(模式菌株VKM Y-2954 = CBS 10874)和隐球菌丝状变种膜状隐球菌(模式菌株VKM Y-2955 = CBS 10875)的名称。