糖蛋白清除速度快，并被鸡胚中的甘露聚糖所抑制。

Glycoprotein clearance is rapid and suppressed by mannan in chicken embryos.

机构信息

Department of Biochemistry and Molecular Biology, College of Biology Science, China Agricultural University, No. 2 Yuanmingyuan West Road, 100193, Beijing, China.

出版信息

J Physiol Biochem. 2009 Sep;65(3):235-41. doi: 10.1007/BF03180576.

DOI:10.1007/BF03180576

PMID:20119818

Abstract

Carbohydrates are thought to function as tags that mark circulatory glycoproteins for rapid clearance. Scavenger endothelial cells (SECs) play the primary role in clearing glycoproteins via receptor-mediated endocytosis in adult animals. We found that horseradish peroxidase (HRP), a glycoprotein, was removed quickly, mostly by receptor mediation from the chicken embryo circulation, but bovine serum albumin was not. The half-life of HRP in the circulation varied with the embryo stage and fell rapidly from 0.73 h at embryonic day 4 (E4) to 0.23 h at E5, with no great difference among stages after E5. HRP clearance was far slower at E3.5 than at E5, but was obviously suppressed by mannan. These results imply that the function of clearing glycoprotein or waste macromolecules from the circulation via receptor-mediated endocytosis appears early in the embryo.

摘要

碳水化合物被认为是一种标记，可快速清除循环糖蛋白。清道夫内皮细胞（SECs）在成年动物中通过受体介导的内吞作用发挥清除糖蛋白的主要作用。我们发现，辣根过氧化物酶（HRP）是一种糖蛋白，它主要通过受体介导从鸡胚循环中迅速清除，但牛血清白蛋白则不会。HRP 在循环中的半衰期随胚胎阶段而变化，从胚胎第 4 天（E4）的 0.73 小时迅速降至 E5 的 0.23 小时，E5 之后各阶段之间没有太大差异。E3.5 时 HRP 的清除速度明显比 E5 时慢，但甘露聚糖明显抑制了其清除。这些结果表明，通过受体介导的内吞作用从循环中清除糖蛋白或废物大分子的功能在胚胎早期就出现了。