Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Mycologia. 2010 Jan-Feb;102(1):83-92. doi: 10.3852/07-147.
The poorly known marine ascomycete Manglicola guatemalensis from Trang and Trat provinces, Thailand, were collected in 2005 and 2006 on the brackish water palm Nypa fruticans. This fungus is known only from two previous collections. This paper reports on the morphological characteristics and molecular phylogeny of this unique marine bitunicate ascomycete. Manglicola guatemalensis has large clavate to obtusely fusiform ascomata, wide ostioles, bitunicate asci, cylindrical, thick-walled, unequally one-septate ascospores, constricted at the septum, apical cell larger, chestnut-brown and a smaller light brown basal cell. Ascospores germinate readily, always from the basal cell. Four isolates from different locations were selected for the phylogenetic study. Regions of the rDNA gene, including SSU and LSU, were sequenced and combined. Molecular data places M. guatemalensis in the Jahnulales with high bootstrap support; all strains are monophyletic. In the combined SSU and LSU analyses the Jahnulales comprises four subclades, A, B, C and D. Subclade A comprises Jahnula species and two anamorphic fungi, Brachiosphaera tropicalis and Xylomyces chlamydosporus. In subclade B Manglicola strains form a sister group of the Aliquandostipite species Aliquandostipite crystallinus, A. khaoyaiensis, Jahnula siamensiae and Patescospora separans. Subclade C comprises Jahnula species, Jahnula aquatica, J. granulosa and J. rostrata, while Megalohypha aqua-dulces constitutes subclade D. Therefore Manglicola forms another lineage of marine fungi.
来自泰国桐艾府和尖竹汶府的海洋子囊菌 Manglicola guatemalensis 于 2005 年和 2006 年在半咸水棕榈 Nypa fruticans 上采集。这种真菌仅从前两次收集到。本文报道了这种独特的海洋双壁子囊菌的形态特征和分子系统发育。Manglicola guatemalensis 具有大型棍棒状至钝梭形子囊果、宽口孔、双壁子囊、圆柱形、厚壁、不均匀的一隔膜子囊孢子、在隔膜处缢缩、顶细胞较大、栗棕色和较小的浅棕色基细胞。子囊孢子容易萌发，总是从基细胞开始。从不同地点选择了四个分离株进行系统发育研究。包括 SSU 和 LSU 的 rDNA 基因区域被测序并组合。分子数据将 M. guatemalensis 置于 Jahnulales 中，支持度很高；所有菌株都是单系的。在联合的 SSU 和 LSU 分析中，Jahnulales 包含四个亚分支，A、B、C 和 D。亚分支 A 包含 Jahnula 物种和两种无性真菌，Brachiosphaera tropicalis 和 Xylomyces chlamydosporus。在亚分支 B 中，Manglicola 菌株与 Aliquandostipite 物种 Aliquandostipite crystallinus、A. khaoyaiensis、Jahnula siamensiae 和 Patescospora separans 形成姐妹群。亚分支 C 包含 Jahnula 物种、Jahnula aquatica、J. granulosa 和 J. rostrata，而 Megalohypha aqua-dulces 构成亚分支 D。因此，Manglicola 形成了另一个海洋真菌谱系。
