Department of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, PR China.
J Microencapsul. 2010;27(2):133-41. doi: 10.3109/02652040903052010.
By a double emulsion solvent evaporation method, interferon-alpha (IFN-alpha) microspheres were prepared with poly(lactide-co-glycolide) (PLGA) and their characteristics, such as morphology, drug loading, encapsulation efficiency, in vitro release and degradation were evaluated. The IFN-alpha microspheres were prepared by different viscosities from 0.17-1.13 dL g(-1) and concentrations between 5-25% of PLGA, which not only affected the drug loading and encapsulation efficiency of IFN-alpha microspheres, but also strongly influenced the in vitro release. With smooth and porous surface, the drug loading and encapsulation efficiency of the microspheres prepared by 15% 0.89 dL g(-1) PLGA were 7.736% and 77.38%, respectively. The DSC curve of microspheres indicated IFN-alpha was loaded inside the microspheres. The degradation of microspheres was homogeneous and the mass loss was over 80% in 6 weeks. The release profile of microspheres showed a sustained fashion and the IFN-alpha released from microspheres maintained its bioactivity for 7 days.
采用复乳溶剂挥发法，以聚乳酸-羟基乙酸共聚物（PLGA）为载体材料制备了干扰素-α（IFN-α）微球，并对其形态、载药量、包封率、体外释放和降解等性能进行了评价。IFN-α微球是由不同黏度（0.17-1.13 dL g(-1))和浓度（5-25%）的 PLGA 制备而成，这不仅影响 IFN-α微球的载药量和包封率，而且强烈影响其体外释放。通过 15% 0.89 dL g(-1)PLGA 制备的微球具有光滑多孔的表面，其载药量和包封率分别为 7.736%和 77.38%。微球的 DSC 曲线表明 IFN-α被载入微球内部。微球的降解是均匀的，在 6 周内质量损失超过 80%。微球的释放曲线呈现持续释放的方式，微球中释放的 IFN-α在 7 天内保持其生物活性。
