Institute of Genetics and Animal Breeding, Wólka Kosowska, Poland.
Biochem Genet. 2010 Jun;48(5-6):450-64. doi: 10.1007/s10528-009-9328-1. Epub 2010 Feb 2.
Myogenic factor 5 (Myf5), a product of the Myf5 gene, belongs to the MRF family of basic helix-loop-helix transcription factors that regulate myogenesis. Their roles in muscle growth and development make their genes candidates for molecular markers of meat production in livestock, but nucleotide sequence polymorphism has not been thoroughly studied in MRF genes. We detected four single nucleotide polymorphisms (SNPs) within exon 1 of the Myf5 gene, encoding the NH-terminal transactivation domain of the Myf5 protein. Three of these mutations change the amino acid sequence. The distribution of these SNPs was highly skewed in cattle populations; most of the mutations were found in only a few or even single individuals. Of the nine SNPs found in the promoter region of Myf5, one (transversion g.-723G-->T) was represented by all three genotypes distributed in the cattle populations studied. This polymorphism showed an influence on Myf5 gene expression in the longissimus dorsi muscle and was associated with sirloin weight and fat weight in sirloin in carcasses of Holstein-Friesian cattle.
肌生成因子 5（Myf5）是 Myf5 基因的产物，属于碱性螺旋-环-螺旋转录因子家族，该家族调节肌发生。它们在肌肉生长和发育中的作用使它们的基因成为家畜肉质生产的分子标记的候选者，但在 MRF 基因中，核苷酸序列多态性尚未得到彻底研究。我们在 Myf5 基因的外显子 1 中检测到了四个单核苷酸多态性（SNP），这些 SNP 编码 Myf5 蛋白的 NH2-端转录激活结构域。其中三个突变改变了氨基酸序列。这些 SNP 在牛群体中的分布高度偏斜；大多数突变只存在于少数甚至单个个体中。在 Myf5 启动子区域发现的 9 个 SNP 中，一个（转换 g.-723G-->T）由分布在研究牛群中的三种基因型表示。这种多态性对背最长肌中的 Myf5 基因表达有影响，并与荷斯坦-弗里森牛胴体里脊的里脊重量和脂肪重量有关。
