豚鼠血浆脂蛋白-载脂蛋白随膳食胆固醇的变化

Changes in the plasma lipoprotein-apoproteins of guinea pigs in response to dietary cholesterol.

作者信息

Guo L S, Meng M, Hamilton R L, Ostwald R

出版信息

Biochemistry. 1977 Dec 27;16(26):5807-12. doi: 10.1021/bi00645a026.

PMID:201278

Abstract

The major apoproteins from four plasma lipoproteins were isolated from control and cholesterol-fed guinea pigs. Apoproteins were studied by column chromatography, polyacrylamide gel electrophoresis, and amino acid analysis. Dietary cholesterol altered the plasma apolipoproteins mainly by an enrichment in the content of arginine-rich polypeptide (ARP) in all density fractions. This protein had a similar molecular weight (34 000), electrophoretic mobility, amino acid composition, and microheterogeneity as ARP reported in other mammalian species. The estimation of plasma concentration of ARP indicates a higher correlation coefficient with plasma unesterified cholesterol (r = 0.98) compared with cholesterol esters (r = 0.62).

摘要

从对照和喂食胆固醇的豚鼠中分离出四种血浆脂蛋白的主要载脂蛋白。通过柱色谱、聚丙烯酰胺凝胶电泳和氨基酸分析对载脂蛋白进行了研究。膳食胆固醇主要通过增加所有密度组分中富含精氨酸的多肽（ARP）含量来改变血浆载脂蛋白。该蛋白质的分子量（34000）、电泳迁移率、氨基酸组成和微异质性与其他哺乳动物物种中报道的ARP相似。ARP血浆浓度的估计表明，与胆固醇酯（r = 0.62）相比，其与血浆未酯化胆固醇的相关系数更高（r = 0.98）。