Castaing M, Kraus J L, Ponge C
Centre de Biochimie et de Biologie Moléculaire, CBM2/CNRS, Marseille, France.
Biophys Chem. 1991 Jan;39(1):17-29. doi: 10.1016/0301-4622(91)85003-9.
The leakage of Ru(bpy)3(2+) across a membrane of dihexadecyl phosphate (DHP) vesicles was compared when induced by (2.1.1), (2.2.1), (2.2.2.), (2.2.1.)C10 and (2.2.2.)C10-cryptands, and by (2.2.) and (2.2.)-bishydroxyethyl, i.e., ionizable macrobicyclic and monocyclic amino polyethers. Ru(bpy)3(2+) leakage increased as the permeant concentrations rose and was much higher for the very lipophilic cryptands. It also increased as the pH fell, and was lower and less dependent on the permeant concentration when induced by addition of partially titrated than by non-pretitrated cryptand. The efficiency of the permeant decreased as the alkali cation concentration rose and was independent of the cation type. It also varied with the membrane type: the efficiency of the (2.2.2.)C10-cryptand was higher on permeation of the membrane of DHP vesicles made from dihexadecyl phosphate than that of large unilamellar vesicles (LUV) composed of alpha-phosphatidylcholine, alpha-phosphatidic acid and cholesterol in an 8:1:1 molar ratio. The results are discussed in terms of the structural, physico-chemical and electrical characteristics of the permeating agents and of the membranes.
比较了由（2.1.1）、（2.2.1）、（2.2.2）、（2.2.1）C10和（2.2.2）C10 - 穴状配体，以及由（2.2）和（2.2） - 双羟乙基，即可电离的大环和单环氨基聚醚诱导时，Ru(bpy)3(2+)跨磷酸二己酯（DHP）囊泡膜的泄漏情况。随着渗透剂浓度升高，Ru(bpy)3(2+)泄漏增加，对于非常亲脂的穴状配体，泄漏量要高得多。随着pH值下降，泄漏量也增加，并且与通过添加部分滴定的穴状配体诱导相比，通过未预滴定的穴状配体诱导时，泄漏量更低且对渗透剂浓度的依赖性更小。随着碱金属阳离子浓度升高，渗透剂的效率降低，且与阳离子类型无关。它还随膜类型而变化：（2.2.2）C10 - 穴状配体在由磷酸二己酯制成的DHP囊泡膜上的渗透效率高于由α - 磷脂酰胆碱、α - 磷脂酸和胆固醇以8:1:1摩尔比组成的大单层囊泡（LUV）膜。根据渗透剂和膜的结构、物理化学及电学特性对结果进行了讨论。
