Serviço de Imunologia, Hospital Universitário Prof Edgard Santos, Universidade Federal da Bahia, Salvador, BA, Brazil.
Clin Exp Immunol. 2010 May;160(2):266-74. doi: 10.1111/j.1365-2249.2009.04084.x. Epub 2010 Feb 2.
Schistosoma mansoni infection has been associated with protection against allergies. The mechanisms underlying this association may involve regulatory cells and cytokines. We evaluated the immune response induced by the S. mansoni antigens Sm22.6, PIII and Sm29 in a murine model of ovalbumin (OVA)-induced airway inflammation. BALB/c mice were sensitized with subcutaneously injected OVA-alum and challenged with aerolized OVA. Mice were given three doses of the different S. mansoni antigens. Lung histopathology, cellularity of bronchoalveolar lavage (BAL) and eosinophil peroxidase activity in lung were evaluated. Immunoglobulin (Ig)E levels in serum and cytokines in BAL were also measured. Additionally, we evaluated the frequency of CD4+forkhead box P3 (FoxP3)+ T cells in cultures stimulated with OVA and the expression of interleukin (IL)-10 by these cells. The number of total cells and eosinophils in BAL and the levels of OVA-specific IgE were reduced in the immunized mice. Also, the levels of IL-4 and IL-5 in the BAL of mice immunized with PIII and Sm22.6 were decreased, while the levels of IL-10 were higher in mice immunized with Sm22.6 compared to the non-immunized mice. The frequency of CD4+FoxP3+ T cells was higher in the groups of mice who received Sm22.6, Sm29 and PIII, being the expression of IL-10 by these cells only higher in mice immunized with Sm22.6. We concluded that the S. mansoni antigens used in this study are able to down-modulate allergic inflammatory mediators in a murine model of airway inflammation and that the CD4+FoxP3+ T cells, even in the absence of IL-10 expression, might play an important role in this process.
曼氏血吸虫感染与过敏保护有关。这种关联的机制可能涉及调节细胞和细胞因子。我们在卵清蛋白（OVA）诱导的气道炎症的小鼠模型中评估了 S. mansoni 抗原 Sm22.6、PIII 和 Sm29 诱导的免疫反应。BALB/c 小鼠通过皮下注射 OVA-明矾进行致敏，并通过雾化 OVA 进行挑战。给小鼠三种不同的 S. mansoni 抗原。评估肺组织病理学、支气管肺泡灌洗液（BAL）细胞数和肺中嗜酸性粒细胞过氧化物酶活性。还测量血清中的免疫球蛋白（Ig）E 水平和 BAL 中的细胞因子。此外，我们评估了在 OVA 刺激下培养物中 CD4+叉头框 P3（FoxP3）+T 细胞的频率以及这些细胞中白细胞介素（IL）-10 的表达。免疫小鼠 BAL 中的总细胞和嗜酸性粒细胞数量以及 OVA 特异性 IgE 水平降低。此外，用 PIII 和 Sm22.6 免疫的小鼠 BAL 中的 IL-4 和 IL-5 水平降低，而用 Sm22.6 免疫的小鼠的 IL-10 水平高于未免疫的小鼠。在接受 Sm22.6、Sm29 和 PIII 的小鼠组中，CD4+FoxP3+T 细胞的频率更高，而这些细胞中仅 IL-10 的表达在 Sm22.6 免疫的小鼠中更高。我们得出结论，本研究中使用的 S. mansoni 抗原能够下调气道炎症的小鼠模型中的过敏炎症介质，即使缺乏 IL-10 表达，CD4+FoxP3+T 细胞也可能在该过程中发挥重要作用。
