Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Shihung-si 429-793, Korea.
J Microbiol Biotechnol. 2010 Jan;20(1):82-7.
Biotransformation is often used to improve chemical activity. We evaluated the antimicrobial activity of rhapontigenin, converted from rhapontin after treatment with Pectinex. Rhapontigenin showed 4-16 times higher antimicrobial activity than rhapontin. Activity was higher against Gram positive strains than Gram negative strains. Minimum inhibitory concentrations (MICs) of rhapontigenin, retinol, and five antibiotics were determined by microbroth dilution method for antibiotic-sensitive and -resistant Propionibacterium acnes. We also investigated the in vitro antibacterial activity of rhapontigenin in combination with antibiotic against antibiotic-resistant P. acnes. The antibiotic combination effect against resistant P. acnes was studied by checkerboard method. The combination formulations (rhapontigenin and clindamycin, retinol and clindamycin) showed synergic effects on the inhibition of the growth of clindamycin-resistant P. acnes. It is predictable that the combination of antibiotics with rhapontigenin is helpful to treat acne caused by antibiotic resistant P. acnes. The antibacterial activity of rhapontigenin was enhanced by biotransformation.
生物转化常用于提高化学活性。我们评估了经过 Pectinex 处理后从瑞普汀转化而来的瑞普酮的抗菌活性。瑞普酮的抗菌活性比瑞普汀高 4-16 倍。它对革兰氏阳性菌株的活性高于革兰氏阴性菌株。采用微量肉汤稀释法测定瑞普酮、视黄醇和五种抗生素对青霉素敏感和耐药痤疮丙酸杆菌的最小抑菌浓度（MIC）。我们还研究了瑞普酮与抗生素联合治疗抗生素耐药痤疮丙酸杆菌的体外抗菌活性。采用棋盘法研究了抗生素组合对耐药痤疮丙酸杆菌的协同作用。瑞普酮与克林霉素、视黄醇与克林霉素的组合制剂对抑制克林霉素耐药痤疮丙酸杆菌的生长具有协同作用。可以预测，抗生素与瑞普酮的联合使用有助于治疗由抗生素耐药痤疮丙酸杆菌引起的痤疮。生物转化增强了瑞普酮的抗菌活性。
