Billinghurst R Clark, Ionescu Mirela, Poole A Robin
School of Applied Sciences, St. Lawrence College, Kingston, ON, Canada.
Methods Mol Biol. 2010;622:349-66. doi: 10.1007/978-1-60327-299-5_21.
This chapter describes the production and characterization of antibodies raised against neoepitopes in collagenase-cleaved collagen. It also details the development, validation, and use of immunoassays using such antibodies to measure specifically collagenase-mediated cleavage.
本章描述了针对胶原酶切割胶原蛋白中的新表位产生的抗体的制备和特性。它还详细介绍了使用此类抗体进行免疫测定以特异性测量胶原酶介导的切割的开发、验证和应用。
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