曼氏血吸虫蛋白减轻实验性结肠炎小鼠的胃肠动力障碍。
Schistosoma mansoni proteins attenuate gastrointestinal motility disturbances during experimental colitis in mice.
机构信息
Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, 2610 Antwerp, Belgium.
出版信息
World J Gastroenterol. 2010 Feb 14;16(6):703-12. doi: 10.3748/wjg.v16.i6.703.
AIM
To investigate the therapeutic effect of Schistosoma mansoni (S. mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice.
METHODS
Colitis was induced by intrarectal injection of trinitrobenzene sulphate (TNBS) and 6 h later, mice were treated ip with S. mansoni proteins. Experiments were performed 5 d after TNBS injection. Inflammation was quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility. Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up. Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction.
RESULTS
Intracolonic injection of TNBS caused severe colitis. Treatment with S. mansoni proteins significantly ameliorated colonic inflammation after 5 d. TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis. Treatment with S. mansoni proteins ameliorated these in vivo and in vitro motility disturbances. In addition, TNBS injection caused a downregulation of effector T cell cytokines after 5 d, whereas a S. mansoni protein effect was no longer observed at this time point.
CONCLUSION
Treatment with S. mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis.
目的
研究曼氏血吸虫可溶性虫体蛋白对实验性结肠炎小鼠胃肠动力紊乱的治疗作用。
方法
采用直肠内注射三硝基苯磺酸（TNBS）诱导结肠炎，6 小时后用曼氏血吸虫蛋白进行腹腔内治疗。在 TNBS 注射后 5 天进行实验。采用验证的炎症参数量化炎症。通过测量胃排空和几何中心来评估体内胃肠动力。使用改良的 Trendelenburg 装置在体外研究远端结肠段的蠕动活动。通过实时逆转录聚合酶链反应测定从结肠分离的 T 淋巴细胞的细胞因子谱。
结果
经结肠内注射 TNBS 可导致严重的结肠炎。在 5 天后，用曼氏血吸虫蛋白治疗可显著改善结肠炎症。TNBS 不影响胃排空，但在诱导结肠炎后 5 天显著降低几何中心并损害结肠蠕动活动。用曼氏血吸虫蛋白治疗可改善这些体内和体外的运动障碍。此外，TNBS 注射后 5 天引起效应 T 细胞细胞因子下调，而此时不再观察到曼氏血吸虫蛋白的作用。
结论
用曼氏血吸虫蛋白治疗可减轻实验性结肠炎小鼠的肠道炎症并改善运动障碍。
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