Xiao Guang-Fen, Tang Xue-Yuan, Li Xin, Zeng Can
Department of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Feb;18(1):15-8.
This study was purposed to investigate the expression and significance of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in bone marrow mononuclear cells (BMMNC) isolated from patients with acute myeloid leukemia (AML), GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase (PLAP) as a substrate and Triton X-114 phase partitioning. The GPI-PLD mRNA expression was measured by semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the mRNA expression level and activity of GPI-PLD in BMMNC from de novo AML patients were 1.86 +/- 0.32 and 46.96 +/- 7.15% respectively; the mRNA expression level and activity of GPI-PLD in BMMNC from completely remission and refractory or relapsed patients were 1.26 +/- 0.29, 33.36 +/- 5.13%and 1.79 +/- 0.19, 44.31 +/- 7.22%, while those in BMMNC from normal controls were 1.27 +/- 0.23, 35.38 +/- 5.15% respectively. The mRNA expression level and activity of GPI-PLD in de novo and refractory or relapsed patients were obviously higher than those in normal controls with significant difference (p < 0.01), while the comparison between remitted patients and normal controls showed no statistical difference (p > 0.05). It is concluded that the expression level of GPI-PLD mRNA coincides with GPI-PLD activity. The mRNA expression and activity of GPI-PLD in de novo and refractory or relapsed patients are obviously higher than those in normal controls. It is worthy of further exploring whether GPI-PLD plays a certain role in process of leukemia pathogenesis.
本研究旨在探讨糖基磷脂酰肌醇特异性磷脂酶D（GPI-PLD）在急性髓系白血病（AML）患者分离的骨髓单个核细胞（BMMNC）中的表达及意义。采用以糖基磷脂酰肌醇锚定的胎盘碱性磷酸酶（PLAP）为底物，通过Triton X-114相分离法，检测了78例AML患者及15例正常人分离的BMMNC中的GPI-PLD活性。采用半定量逆转录聚合酶链反应（RT-PCR）检测GPI-PLD mRNA表达。结果显示，初发AML患者BMMNC中GPI-PLD的mRNA表达水平及活性分别为1.86±0.32和46.96±7.15%；完全缓解及难治或复发患者BMMNC中GPI-PLD的mRNA表达水平及活性分别为1.26±0.29、33.36±5.13%和1.79±0.19、44.31±7.22%，而正常对照者BMMNC中分别为1.27±0.23、35.38±5.15%。初发及难治或复发患者GPI-PLD的mRNA表达水平及活性明显高于正常对照者，差异有统计学意义（p<0.01），而缓解患者与正常对照者比较无统计学差异（p>0.05）。结论：GPI-PLD mRNA表达水平与GPI-PLD活性一致。初发及难治或复发患者GPI-PLD的mRNA表达及活性明显高于正常对照者。GPI-PLD在白血病发病过程中是否发挥一定作用值得进一步探讨。
