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瑞典奶牛源隐孢子虫分离株的分子特征与年龄、腹泻和地区的关系。

Molecular characterisation of Cryptosporidium isolates from Swedish dairy cattle in relation to age, diarrhoea and region.

机构信息

Department of Clinical Sciences, Division of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, P.O. Box 7054, SE-750 07 Uppsala, Sweden.

出版信息

Vet Parasitol. 2010 May 11;169(3-4):289-95. doi: 10.1016/j.vetpar.2010.01.003. Epub 2010 Jan 20.

Abstract

Cryptosporidium positive samples from 176 preweaned calves, young stock and cows of 48 herds were subjected to molecular characterisation of the 18S rRNA gene to determine which species are present in Swedish dairy cattle. In addition, samples characterised as Cryptosporidium parvum were further analysed at the GP60 gene to investigate distribution and zoonotic potential of subtypes. The 18S rRNA gene was successfully sequenced in 110 samples, with Cryptosporidium bovis in 83, C. parvum in 15, Cryptosporidium ryanae in 10, and Cryptosporidium andersoni in two samples. C. bovis was the most common species, being identified in 74% of calf samples, in 77% of young stock samples and in 100% of cow samples. The youngest calves infected with C. bovis were 7 days old, showing that the prepatent period is shorter than the previously stated 10 days. C. parvum was detected in 15 calves from nine farms, and samples were clustered in the southern parts of Sweden. Diarrhoeic calf samples contained C. parvum, C. bovis or C. ryanae. Sequencing of the GP60 gene was successful in 13 of the C. parvum samples. Eight subtypes, including three novel ones, were detected. Four of the subtypes have previously been identified in humans. This indicates that there is a zoonotic potential in C. parvum infected Swedish dairy calves.

摘要

对来自 48 个牛群的 176 头未断奶小牛、幼畜和奶牛的 18S rRNA 基因进行分子特征分析,以确定在瑞典奶牛中存在哪些物种。此外,进一步对鉴定为小隐孢子虫的样本进行 GP60 基因分析,以研究亚型的分布和人畜共患潜力。成功对 110 个样本进行了 18S rRNA 基因测序,其中 83 个样本为牛隐孢子虫,15 个样本为小隐孢子虫,10 个样本为赖氏隐孢子虫,2 个样本为安德森隐孢子虫。牛隐孢子虫是最常见的物种,在 74%的小牛样本、77%的幼畜样本和 100%的奶牛样本中均有发现。感染牛隐孢子虫的最小小牛为 7 天大,表明其潜伏期比之前所述的 10 天要短。在来自 9 个农场的 15 头小牛中检测到小隐孢子虫,样本在瑞典南部地区聚集。患有腹泻的小牛样本中含有小隐孢子虫、牛隐孢子虫或赖氏隐孢子虫。成功对 13 个小隐孢子虫样本进行了 GP60 基因测序。检测到 8 个亚型,包括 3 个新亚型。其中 4 个亚型之前已在人类中发现。这表明感染小隐孢子虫的瑞典奶牛幼畜具有人畜共患的潜力。

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