Laboratory of Cell Metabolism, Department of Pharmaceutical Sciences, K.U. Leuven, Leuven, Belgium.
FEBS Lett. 2010 Mar 5;584(5):1054-8. doi: 10.1016/j.febslet.2010.01.061. Epub 2010 Feb 9.
aP2-Cre mice have amply been used to generate conditional adipose selective inactivation of important signaling molecules. We show that the efficiency of Cre mediated recombination in adipocytes and adipose selectivity is not always guaranteed. In particular, Cre activity was found in ganglia of the peripheral nervous system (PNS), in adrenal medulla and in neurons throughout the central nervous system (CNS). Because these tissues have an important impact on adipose tissue, care should be taken when using aP2-Cre mice to define the role of the targeted genes in adipose tissue function.
aP2-Cre 小鼠已被广泛用于生成条件性脂肪组织中重要信号分子的选择性失活。我们发现，Cre 介导的重组在脂肪细胞中的效率和脂肪组织的选择性并非总是得到保证。特别是，在周围神经系统（PNS）的神经节、肾上腺髓质和中枢神经系统（CNS）的神经元中发现了 Cre 活性。由于这些组织对脂肪组织有重要影响，因此在使用 aP2-Cre 小鼠来确定靶基因在脂肪组织功能中的作用时应谨慎。
