Instituto de Pesquisas Biológicas, Laboratório Central de Saúde Pública do Rio Grande do Sul, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
Mem Inst Oswaldo Cruz. 2009 Dec;104(8):1072-82. doi: 10.1590/s0074-02762009000800003.
Eggs and nymphs of Triatoma dimidiata were described using both light and scanning electron microscopy. The egg body and operculum have an exochorion formed by irregular juxtaposed polygonal cells; these cells are without sculpture and the majority of them are hexagonal in shape. The five instars of T. dimidiatacan be distinguished from each other by characteristics of the pre, meso and metanotum. The number of setiferous tubercles increases progressively among instars. The sulcus stridulatorium of 1st instar nymphs is amorphous, showing median parallel grooves; from the 2nd instar on the sulcus is, progressively, elongate, deep and posteriorly pointed with stretched parallel grooves. All instars have a trichobothrium on the apical 1/3 of segment II of the antenna. The opening of the Brindley's gland is on the mesopleura. Fifth instar nymphs have an apical ctenidium on the ventral surface of the fore tibia. Dorsal glabrous patches are found on the lateral 1/3 of abdomen. Bright oval patches are found on the ventral median line of the abdomen, from segment IV-VI; 1st instar nymphs lack these patches. Abdominal dorsal plates are present from the 1st-5th instars; the 1st instar also contains a rectangular plate in segment IX. From the 2nd instar on, variably-shaped plates are present on segments VII to IX. Morphometric data were also obtained and proved to be useful for distinguishing T. dimidiata instars.
使用光学显微镜和扫描电子显微镜对 Triatoma dimidiata 的卵和若虫进行了描述。卵体和卵盖具有由不规则并置的多边形细胞形成的外表皮;这些细胞没有纹饰,大多数呈六边形。T. dimidiatacan 的五个龄期可以通过前胸、中胸和后胸的特征来区分。刚毛瘤的数量在各龄期逐渐增加。1 龄若虫的摩擦音沟是无定形的,显示出中央平行的凹槽;从 2 龄开始,摩擦音沟逐渐变长、加深,并向后变尖,具有伸展的平行凹槽。所有龄期的触角节 II 的顶端 1/3 都有一根纤毛。Brindley 腺的开口位于中胸侧板上。5 龄若虫在前胫节腹面有一个顶端小刺。腹部侧面 1/3 有光裸区。腹部腹中线有明亮的椭圆形斑块,从第 4 到第 6 节;1 龄若虫缺乏这些斑块。腹部背面板从 1 龄到 5 龄都存在;1 龄还在第 9 节有一个矩形板。从 2 龄开始,7 到 9 节上有形状不同的板。还获得了形态计量学数据,这些数据对于区分 T. dimidiata 的龄期很有用。