Department of Pharmaceutical Technology, Faculty of Pharmacy, Medical University of Bialystok, 15-222 Bialystok, Poland.
Nat Prod Res. 2010 Feb;24(3):274-85. doi: 10.1080/14786410902991878.
In this study, we looked at the effect of ouabain, digoxin and proscillaridin A on human fibroblasts. These data show that low concentrations of ouabain, digoxin and proscillaridin A can activate proliferation of human fibroblasts, suggesting that the Na+, K+-adenosine triphosphatase complex may act as a transducing receptor. It was shown that 30 nM ouabain, digoxin and proscillaridin A stimulated an antiapoptotic action by the increase in the level of phosphorylated extracellular signal-regulated kinases (P-ERK 1/2). Ouabain, digoxin and proscillaridin A only at the relatively high concentration of 300 nM increased intracellular Ca2+ concentration, activated caspase-3 and induced apoptosis in human fibroblasts. In terms of reduction in cell viability, antiproliferative and apoptotic activity, these cardiac glycosides rank in the order proscillaridin A >digoxin >ouabain.
在这项研究中，我们观察了哇巴因、地高辛和毛花苷丙对人成纤维细胞的影响。这些数据表明，低浓度的哇巴因、地高辛和毛花苷丙可以激活人成纤维细胞的增殖，这表明 Na+，K+-三磷酸腺苷酶复合物可能作为一种转导受体发挥作用。结果表明，30 nM 的哇巴因、地高辛和毛花苷丙通过增加磷酸化细胞外信号调节激酶（P-ERK1/2）的水平刺激抗细胞凋亡作用。哇巴因、地高辛和毛花苷丙仅在相对较高的 300 nM 浓度下增加细胞内 Ca2+浓度，激活半胱天冬酶-3 并诱导人成纤维细胞凋亡。就细胞活力降低、抗增殖和促凋亡活性而言，这些强心苷的顺序为毛花苷丙>地高辛>哇巴因。
