Zhao Yaping, Wang Jialing, Cai Youqun, Chen Xiaohui, Zhang Chunmei, Guo Xirong
Department of Endocrinology, the 82nd Hospital of the People's Liberation Army, Huaian, Jiangsu, 223001 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Feb;27(1):69-72. doi: 10.3760/cma.j.issn.1003-9406.2010.01.015.
To observe the changes of NYGGF4 gene expression during human preadipocyte differentiation and to explore the regulative role of tumor necrosis factor alpha (TNFalpha) on NYGGF4 gene expression in matured human adipocytes.
Human preadipocytes-visceral (HPA-v) were cultured in vitro and differentiated into the matured adipocytes. Fully differentiated adipocytes were treated with TNFalpha in different concentrations for 16 hours or at 10 ng/mL for various times. Levels of the NYGGF4 mRNA were evaluated by real-time reverse transcription-PCR(RT-PCR).
(1) After induction of differentiation, more than 90% of the HPAs-v exhibited typical adipocyte morphology on the 17th day. (2) In human preadipocytes, the level of NYGGF4 mRNA expression remained low. The NYGGF4 mRNA level was gradually increased with the preadipocytes being differentiated into the matured adipocytes, and reached the highest level in the fully differentiated adipocytes. (3) Treatment of the human matured adipocytes with TNFalpha resulted in a significant increase in the level of NYGGF4 mRNA. The up-regulatory effect of TNFalpha on the NYGGF4 gene expression tended to be enhanced with the increasing concentrations and elongation of time.
During human preadipocytes differentiation, the level of NYGGF4 gene mRNA expression increases gradually. TNFalpha could upregulate the expression of NYGGF4 mRNA in human matured adipocytes. The regulatory effect of TNFalpha on the NYGGF4 gene expression is of dose-dependent and time-correlated.
观察NYGGF4基因在人前脂肪细胞分化过程中的表达变化，探讨肿瘤坏死因子α（TNFα）对人成熟脂肪细胞中NYGGF4基因表达的调控作用。
体外培养人内脏前脂肪细胞（HPA-v）并诱导其分化为成熟脂肪细胞。将完全分化的脂肪细胞用不同浓度的TNFα处理16小时，或用10 ng/mL的TNFα处理不同时间。通过实时逆转录聚合酶链反应（RT-PCR）评估NYGGF4 mRNA的水平。
（1）诱导分化后，超过90%的HPA-v在第17天呈现典型的脂肪细胞形态。（2）在人前脂肪细胞中，NYGGF4 mRNA表达水平较低。随着前脂肪细胞分化为成熟脂肪细胞，NYGGF4 mRNA水平逐渐升高，并在完全分化的脂肪细胞中达到最高水平。（3）用TNFα处理人成熟脂肪细胞导致NYGGF4 mRNA水平显著升高。TNFα对NYGGF4基因表达的上调作用随浓度增加和时间延长而增强。
在人前脂肪细胞分化过程中，NYGGF4基因mRNA表达水平逐渐升高。TNFα可上调人成熟脂肪细胞中NYGGF4 mRNA的表达。TNFα对NYGGF4基因表达的调控作用具有剂量依赖性和时间相关性。
