塞来昔布抑制幽门螺杆菌定植相关因子。
Celecoxib inhibits Helicobacter pylori colonization-related factors.
机构信息
Department of Gastroenterology, Peking University First Hospital, 8 Xishiku Avenue, Xicheng District, Beijing 100034, China.
出版信息
World J Gastroenterol. 2010 Feb 21;16(7):846-53. doi: 10.3748/wjg.v16.i7.846.
AIM
To investigate the effect of celecoxib, a selective COX-2 inhibitor, on Helicobacter pylori (H. pylori) colonization-related factors and its mechanism.
METHODS
After co-incubation with celecoxib, morphology of H. pylori strain 26695 was observed under a transmission electron microscope. Flagella motility was assessed by stab agar motility test. Adherence of H. pylori to AGS cells was determined by enzyme linked immunosorbent assay. Levels of mRNA expression in flagellar genes (flaA, flaB), urease genes (ureA, ureB) and adhesin genes (babA, sabA, alpA, alpB, hpaA, hopZ) were measured by real-time polymerase chain reaction.
RESULTS
Separation and non-integrity of bacterial cell wall, rarefaction and asymmetry of cytoplasm, and even lysis of H. pylori were observed in the presence of celecoxib. When H. pylori strains were incubated in the presence of celecoxib, their flagellar motility and adherence to AGS cells were inhibited. The expression of ureA, ureB, babA, sabA, alpA, alpB, hpaA, hopZ was up-regulated while the expression of flaA, flaB was down-regulated in the presence of celecoxib.
CONCLUSION
Celecoxib inhibits flagellar motility and adherence of H. pylori to AGS cells, and destructs their normal structure in vitro.
目的
研究选择性 COX-2 抑制剂塞来昔布对幽门螺杆菌（H. pylori）定植相关因素的影响及其机制。
方法
在与塞来昔布共孵育后，通过透射电子显微镜观察 H. pylori 菌株 26695 的形态。通过穿刺琼脂动力试验评估鞭毛运动。通过酶联免疫吸附试验测定 H. pylori 对 AGS 细胞的黏附。通过实时聚合酶链反应测量鞭毛基因（flaA、flaB）、脲酶基因（ureA、ureB）和黏附素基因（babA、sabA、alpA、alpB、hpaA、hopZ）的 mRNA 表达水平。
结果
在塞来昔布存在的情况下，观察到细菌细胞壁的分离和完整性丧失、细胞质稀疏和不对称，甚至 H. pylori 的裂解。当 H. pylori 菌株在塞来昔布存在下孵育时，其鞭毛运动和对 AGS 细胞的黏附受到抑制。在塞来昔布存在的情况下，ureA、ureB、babA、sabA、alpA、alpB、hpaA、hopZ 的表达上调，而 flaA、flaB 的表达下调。
结论
塞来昔布抑制 H. pylori 的鞭毛运动和对 AGS 细胞的黏附，并在体外破坏其正常结构。
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