[Genetic polymorphisms of 10 STR (AmpFlSTR SGM Plus system) loci in Gypsy population from Poland].

作者信息

Michalczak Ryszard, Kałuzewski Bogdan, Berent Jarosław

机构信息

Wydział Biologii Centralnego Laboratorium Kryminalistycznego Komendy Głównej Policji w Warszawie, Al. Ujazdowskie 7, 00-583 Warszawa.

出版信息

Ann Acad Med Stetin. 2007;53 Suppl 2:136-8.

PMID:20143697

Abstract

INTRODUCTION

The aim of the paper was to create a database of the allele distribution at 10 STR loci (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA).

MATERIAL AND METHODS

The DNA were isolated from samples collected from 222 unrelated individuals of the Gypsy population from Poland. Amplification was performed using a commercial multiplex FlSTR SGM Plus kit. The amplified fragments were resolved by electrophoresis in a 5% denaturing polyacrylamid gel using tne ABI Prism 377 DNA sequencer. The expected performance of the analyzed loci for personal identification testing was estimated.

RESULTS

All loci met the Hardy-Weinberg assumption. The combinated values of the matching probability (MP) and of the power exclusion (PE) are 3.95 x 10(-13) and 0.9999641.

CONCLUSIONS

Statistical parameters (PIC, MP, PE) showed that the examined systems are useful for forensic medicine.

摘要

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