银杏干提取物中萜类内酯测定替代方法的开发。

Development of an alternative method for determination of terpene lactones in ginkgo dry extract.

作者信息

Ekman L, Fransson D, Claeson P, Johansson M

机构信息

Medical Products Agency, SE-751 03 Uppsala, Sweden.

出版信息

Pharmeur Bio Sci Notes. 2009 Oct;2009(1):67-71.

PMID:20144453

Abstract

A new liquid chromatographic mass spectrometric (LC-MS) method for determination of terpene lactones in ginkgo dry extract has been developed. The new method has several advantages over the existing European Pharmacopoeia (Ph. Eur.) method for analysis of terpene lactones in ginkgo dry extract, the major ones being a very simple sample pre-treatment and an excellent selectivity. 5 terpene lactones were analysed with a precision expressed as relative standard deviation (RSD) of 0.4-3.1% and a mean relative error (RE) within +/-4.6%. The method was used to analyse 9 samples of ginkgo dry extracts from 3 different extract producers. The content of bilobalide was found to be in the range of 2.6-3.4% in all samples, whereas the sum of ginkgolides A, B and C was found to be in the range of 3.0-3.6%. Ginkgolide J was found in the range of 0.3-0.6%.

摘要

已开发出一种用于测定银杏干提取物中萜类内酯的新型液相色谱-质谱联用（LC-MS）方法。与现有的欧洲药典（Ph. Eur.）分析银杏干提取物中萜类内酯的方法相比，该新方法具有多个优点，主要优点是样品预处理非常简单且选择性极佳。对5种萜类内酯进行了分析，其精密度以相对标准偏差（RSD）表示为0.4 - 3.1%，平均相对误差（RE）在±4.6%以内。该方法用于分析来自3个不同提取物生产商的9个银杏干提取物样品。发现所有样品中白果内酯的含量在2.6 - 3.4%范围内，而银杏内酯A、B和C的总和在3.0 - 3.6%范围内。银杏内酯J的含量在0.3 - 0.6%范围内。