Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Building 43421, CEP 91501-970 Porto Alegre, Rio Grande do Sul, Brazil.
J Invertebr Pathol. 2010 Jul;104(3):227-30. doi: 10.1016/j.jip.2010.01.012. Epub 2010 Feb 6.
Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a approximately 120 kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots.
表达苏云金芽孢杆菌 Cry 毒素的生物技术作物为昆虫防治提供了一种有价值的方法。Cry8Ka5 对棉铃象鼻虫（Anthonomus grandis）具有高度毒性，被用作研究毒素-配体相互作用的模型。毒素覆盖分析后检测到三种 Cry 结合蛋白。经过从头测序，鉴定出热休克同源蛋白和 V-ATPase，而大约 120 kDa 的蛋白质仍然未知。通过二维凝胶电泳配体印迹进一步观察到其他 Cry8Ka5 结合蛋白。
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