采用大气压化学电离质谱检测的超高效液相色谱法快速分离和测定植物油中的甾醇。
Fast separation and determination of sterols in vegetable oils by ultraperformance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection.
机构信息
Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, E-46100 Burjassot, Valencia, Spain.
出版信息
J Agric Food Chem. 2010 Mar 10;58(5):2771-6. doi: 10.1021/jf9043887.
A method for the determination of sterols in vegetable oils by ultraperformance liquid chromatography (UPLC) with atmospheric pressure chemical ionization mass spectrometry detection has been developed. The separation of sterols was optimized in terms of mobile phase composition, column temperature and flow rate. The optimal conditions were achieved using an Acquity UPLC BEH C18 column (50 x 2.1 mm, 1.7 microm) with a mobile phase consistent of acetonitrile/water (0.01% acetic acid) using a linear gradient, at a flow rate of 0.8 mL min(-1) and column temperature of 10 degrees C, giving a total analysis time below 5 min. The determination was performed in selective ion recording mode. The limits of detection were in all cases below 0.07 microg mL(-1), with relative standard deviation values of retention times and peak areas below 0.4 and 5%, respectively. The content of main sterols present in several vegetable oils with different botanical origins was also established.
建立了一种超高效液相色谱（UPLC）-大气压化学电离质谱（APCI-MS）检测法测定植物油中甾醇的方法。从流动相组成、柱温、流速等方面对甾醇的分离进行了优化。采用 Acquity UPLC BEH C18 柱（50×2.1mm，1.7μm），在 10℃下，以乙腈/水（0.01%乙酸）为流动相，在 0.8mL/min 的流速下进行线性梯度洗脱，总分析时间在 5min 以下，实现了最佳分离条件。采用选择离子记录模式进行检测。在所有情况下，检测限均低于 0.07μg/mL，保留时间和峰面积的相对标准偏差分别低于 0.4%和 5%。还确定了不同植物来源的几种植物油中主要甾醇的含量。
Zotero 插件