Department of Paediatrics, Rheumatology Unit, A. Meyer Children's Hospital, Florence, Italy.
Clin Exp Rheumatol. 2009 Nov-Dec;27(6):1026-30.
To investigate the possible relationship between MIF -173 polymorphism and susceptibility to, and severity of, Kawasaki disease (KD) in a cohort of Italian patients.
Sixty-nine patients (43 F, 26 M, median age 29 months, range 3-135 months) with KD and 60 sex-matched healthy caucasian children were genotyped for MIF-173. Typing of the MIF gene -173 G/C was performed by PCR and restriction fragment length polymorphism.
Eight out of 69 (12%) KD children were non-responders: 7 required an additional IVIG infusion, while 1 received 2 IVIG infusions and then steroid administration. 9/69 (13%) KD children developed coronary artery abnormalities (CAA) during the second to fourth week of disease, and 4 of them required an additional IVIG infusion. MIF genotyping did not show significant differences between patients and controls. KD patients carrying a MIF -173C allele developed CAA more frequently than those without MIF- (7/9 77.8% vs. 16/60 26.7%, OR 9.6, 95% CI 1.80-21.2, p<0.005). Non-responders to a single IVIG infusion carried the MIF -173C allele more frequently than responders (6/8 = 75% vs. 17/61 = 28%, OR 5.1, 95% CI 1.42-22.31 p<0.014). In multiple regression analysis, KD patients carrying a MIF -173*C allele were found to have an increased risk of coronary involvement (OR 7.7, 95% CI 1.36-16.1, p=0.021).
We showed that children carrying the MIF polymorphism -173*C had a higher percentage of CAA. A potential relationship between a MIF polymorphism and risk of CAA in KD might be considered.
研究 MIF-173 多态性与意大利患者川崎病（KD）易感性和严重程度的可能关系。
69 例 KD 患者（43 例女性，26 例男性，中位年龄 29 个月，范围 3-135 个月）和 60 例性别匹配的健康白种儿童进行 MIF-173 基因分型。MIF 基因-173 G/C 的分型通过 PCR 和限制性片段长度多态性进行。
69 例 KD 患儿中有 8 例（12%）为无反应者：7 例需要额外的 IVIG 输注，1 例接受 2 次 IVIG 输注后给予类固醇治疗。69 例 KD 患儿中有 9 例（13%）在疾病的第 2 至第 4 周发生冠状动脉异常（CAA），其中 4 例需要额外的 IVIG 输注。MIF 基因分型在患者和对照组之间没有显示出显著差异。携带 MIF-173C 等位基因的 KD 患者发生 CAA 的频率高于不携带 MIF-（7/9 77.8% vs. 16/60 26.7%，OR 9.6，95%CI 1.80-21.2，p<0.005）。对单次 IVIG 输注无反应的患者携带 MIF-173C 等位基因的频率高于有反应的患者（6/8 = 75% vs. 17/61 = 28%，OR 5.1，95%CI 1.42-22.31，p<0.014）。在多因素回归分析中，携带 MIF-173*C 等位基因的 KD 患者发生冠状动脉受累的风险增加（OR 7.7，95%CI 1.36-16.1，p=0.021）。
我们表明，携带 MIF 多态性-173*C 的儿童 CAA 发生率较高。MIF 多态性与 KD 患者 CAA 风险之间可能存在潜在关系。
