Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, China.
J Chromatogr A. 2010 Mar 12;1217(11):1799-805. doi: 10.1016/j.chroma.2010.01.049. Epub 2010 Jan 25.
A simple, precise, and accurate hydrophilic interaction liquid chromatographic (HILIC) method has been developed for the determination of five aromatic amines in environmental water samples. Chromatography was carried out on a bare silica column, using a mixture of acetonitrile and a buffer of NaH(2)PO(4)-H(3)PO(4) (pH 1.5, containing 10mM NaH(2)PO(4)) (85:15, v/v) as a mobile phase at a flow rate of 1 mL min(-1). Aromatic amines were detected by UV absorbance at 254 nm. The linear range of amines was good (r(2)>0.998) and limit of detection (LOD) within 0.02-0.2 mg L(-1) (S/N=3). The retention mechanism for the analytes under the optimum conditions was determined to be a combination of adsorption, partition and ionic interactions. The proposed method was applied to the environmental water samples. Aromatic amines were isolated from aqueous samples using solid-phase extraction (SPE) with Oasis HLB cartridges. Recoveries of greater than 75% with precision (RSD) less than 12% were obtained at amine concentrations of 5-50 microg L(-1) from 100mL river water and influents from a wastewater treatment plant (WWTP). The present HILIC technique proved to be a viable method for the analysis of aromatic amines in the environmental water samples.
已经开发出一种简单、精确和准确的亲水相互作用液相色谱（HILIC）方法，用于测定环境水样中的五种芳香胺。在裸硅胶柱上进行色谱分离，使用乙腈和 NaH（2）PO（4）-H（3）PO（4）缓冲液（pH 1.5，含 10mM NaH（2）PO（4））（85:15，v/v）的混合物作为流动相，流速为 1 mL min（-1）。通过在 254nm 处的紫外吸光度检测芳香胺。胺的线性范围良好（r（2）>0.998），检测限（LOD）在 0.02-0.2mg L（-1）范围内（S/N=3）。在最佳条件下，分析物的保留机制被确定为吸附、分配和离子相互作用的组合。该方法已应用于环境水样。采用 Oasis HLB 萃取小柱固相萃取（SPE）从水样中分离出芳香胺。在 5-50μg L（-1）的胺浓度下，从 100mL 河水和废水处理厂（WWTP）的进水样中，回收率大于 75%，精密度（RSD）小于 12%。本 HILIC 技术被证明是一种用于分析环境水样中芳香胺的可行方法。
