Sensory & Motor System Medicine, The University of Tokyo, Tokyo, Japan.
Biomaterials. 2010 May;31(14):4009-16. doi: 10.1016/j.biomaterials.2010.01.100. Epub 2010 Feb 10.
Preventing peritendinous adhesions after surgical repair of tendon is difficult. In order to establish an ideal anti-adhesion material, we prepared a spontaneously forming hydrogel by mixing the aqueous solutions of two polymers, poly(MPC-co-methacrylic acid) (PMA) and amphiphilic poly(MPC-co-n-butyl methacrylate) (PMB), in the presence of Fe(3+). This PMA/PMB/Fe(3+) hydrogel (MPC polymer hydrogel) had a honeycomb microstructure with nanometer-scale pores, which resist cell invasion but allow the passage of cytokines and growth factors for tendon healing. The dissociation rate of the hydrogel could be controlled by changing Fe(3+) concentration, and by examining the viscoelasticity of the hydrogel, we determined the optimal Fe(3+) concentration to be 0.05 M. We then examined the effects of the in situ application of this MPC polymer hydrogel containing 0.05 M Fe(3+) by using two animal models: the rat Achilles tendon model and the chicken flexor digitorum profundus tendon model. In both models, macroscopic and histological observation revealed that peritendinous adhesions were significantly decreased by the hydrogel application. Mechanical analyses revealed that the hydrogel prevented peritendinous adhesions but did not affect the tendon healing. Because of its characteristic microstructure and excellent biocompatibility, we believe that the MPC polymer hydrogel will be ideal for preventing peritendinous adhesions.
术后修复肌腱时防止腱周粘连很困难。为了制备理想的防粘连材料，我们在混合了两种聚合物的水溶液，即聚（MPC-共-甲基丙烯酸）（PMA）和两亲性聚（MPC-共-正丁基甲基丙烯酸酯）（PMB）后，通过添加 Fe(3+) 制备了一种自形成水凝胶。这种 PMA/PMB/Fe(3+)水凝胶（MPC 聚合物水凝胶）具有蜂窝状微观结构和纳米级孔，能抵抗细胞侵入，但允许细胞因子和生长因子通过，从而促进肌腱愈合。通过改变 Fe(3+)浓度可以控制水凝胶的解离速率，通过检查水凝胶的粘弹性，我们确定最佳的 Fe(3+)浓度为 0.05 M。然后，我们使用两种动物模型，即大鼠跟腱模型和鸡屈趾深肌腱模型，研究了原位应用含有 0.05 M Fe(3+)的 MPC 聚合物水凝胶的效果。在这两种模型中，宏观和组织学观察都表明水凝胶的应用显著减少了腱周粘连。力学分析表明，水凝胶可防止腱周粘连，但不影响肌腱愈合。由于其独特的微观结构和良好的生物相容性，我们相信 MPC 聚合物水凝胶将是预防腱周粘连的理想材料。
