Department of Pathology and Animal Health, Faculty of Veterinary Medicine, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy.
Vet Parasitol. 2010 May 11;169(3-4):395-8. doi: 10.1016/j.vetpar.2010.01.008. Epub 2010 Jan 20.
The FLOTAC techniques involve the spinning of faecal samples onto the surface of counting chambers to permit enumeration of parasitic elements (eggs, larvae, oocysts and cysts) to an accuracy of one parasitic element per gram of faeces. In the present study it is demonstrated that FLOTAC provides a rapid and very sensitive method for counting of lungworm larvae of sheep. The optimum flotation solution for lungworm larvae is zinc sulphate and mercury II iodide (s.g. 1.45) although zinc sulphate (s.g. 1.20 or 1.35) on its own also gave good results. Samples preserved in 5% formalin gave the highest counts but fresh, frozen and samples in 10% formalin also gave higher counts than McMaster and simple flotation. Larval counts of 307 field samples gave up to 1.27x more positives samples than use of Baermann funnels and up to 4.18x more larvae per sample. As FLOTAC is faster than Baermannisation of samples it offers a better method of counting larvae in ruminant faecal samples.
FLOTAC 技术涉及将粪便样本旋转到计数室的表面上，以允许对寄生虫元素（卵、幼虫、卵囊和胞囊）进行计数，准确度为每克粪便一个寄生虫元素。在本研究中，证明了 FLOTAC 提供了一种快速且非常敏感的方法来计算绵羊的肺蠕虫幼虫。肺蠕虫幼虫的最佳浮选溶液是硫酸锌和碘化汞 II（比重 1.45），尽管单独使用硫酸锌（比重 1.20 或 1.35）也能得到很好的结果。保存在 5％甲醛中的样品给出了最高的计数，但新鲜的、冷冻的和保存在 10％甲醛中的样品比麦克马斯特和简单浮选的计数更高。对 307 个现场样本的幼虫计数，比使用巴氏漏斗获得的阳性样本多了 1.27 倍，每个样本中的幼虫多了 4.18 倍。由于 FLOTAC 比样品的巴氏法更快，因此它为反刍动物粪便样本中的幼虫计数提供了更好的方法。
Zotero 插件