微芯片电泳法用于监测聚乙二醇与蛋白质的共价结合。

Microchip electrophoresis for monitoring covalent attachment of poly(ethylene glycol) to proteins.

机构信息

College of Pharmacy, Kyungsung University, Nam-ku, Busan 608-736, South Korea.

出版信息

Anal Biochem. 2010 May 15;400(2):304-6. doi: 10.1016/j.ab.2010.02.002. Epub 2010 Feb 10.

DOI:10.1016/j.ab.2010.02.002

PMID:20149779

Abstract

A microchip electrophoretic method was applied to monitor and characterize the covalent attachment of poly(ethylene glycol) (PEGylation) of two proteins, alpha-lactalbumin and bovine serum albumin, using several poly(ethylene glycol) (PEG) derivatives with molecular weights from 1 to 20 kDa. This method effectively separated multi-PEGylated proteins in a size-based manner and allowed monitoring of the PEGylation pattern with the advantages of high speed, minimal sample consumption, and high reproducibility. Microchip electrophoresis would be a very useful tool for protein PEGylation studies such as reaction monitoring, purity checks, and characterization of PEGylated protein products.

摘要

一种微芯片电泳方法被应用于监测和描述两种蛋白质（α-乳白蛋白和牛血清白蛋白）的共价连接，即聚乙二醇化（PEGylation），使用了几种分子量从 1 到 20 kDa 的聚乙二醇（PEG）衍生物。该方法可以有效地以尺寸为基础分离多 PEG 化蛋白质，并允许监测 PEG 化模式，具有速度快、样品消耗少、重现性高的优点。微芯片电泳将成为蛋白质 PEG 化研究的一个非常有用的工具，如反应监测、纯度检查和 PEG 化蛋白质产品的特性描述。