某些抗氧化剂对接受L-甲状腺素大鼠肺匀浆脂质过氧化过程的影响。

Effects of certain antioxidants on lipid peroxidation process in lung homogenates of L thyroxine-receiving rats.

作者信息

Wiktorska Joanna A, Lewinski Andrzej, Stuss Michal, Nowak Dariusz, Pietras Tadeusz, Sewerynek Ewa

机构信息

Department of Endocrine Disorders and Bone Metabolism, Medical University of Lodz, Poland.

出版信息

Neuro Endocrinol Lett. 2010;31(1):137-46.

PMID:20150865

Abstract

OBJECTIVE

A possible role of antioxidants in thyreotoxicosis was investigated. We examined the parameters of lipid peroxidation (LPO): conjugated dienes (CD), malondialdehyde (MDA), Schiff bases (SB) in lung homogenates of male Wistar rats.

METHODS

Two control groups were created: Group 1 - intact animals and Group 2 - animals injected with 0,9% NaCl. In Experiment I, the animals received L-thyroxin (LT4) i.p. (Groups 3-7). After one week the rats received additionally: Group 4 - melatonin (MEL); Group 5 - propylthiouracil (PTU); Group 6 - Ambroxol (AMB); Group 7 - N-acetylocysteine (NAC). In Experiment II, the animals received only antioxidants.

RESULTS

In Experiment I, we noticed a significantly higher MDA and SB level in Group 2, compared to that in Group 1. Moreover, we observed a significantly higher MDA and SB level in Group 3, vs. that in Group 1, but SB level was lower in Group 3 than in Group 2. Melatonin, PTU and NAC reduced CD; PTU, AMB diminished MDA and MEL, AMB lowered SB levels as compared to Group 3. In Experiment II, we observed significantly higher MDA and SB level in Group 2, vs. that in Group 1. Melatonin, AMB and NAC decreased MDA and SB level, when compared to Group 2 but PTU elevated MDA and SB level vs. that in Group 1.

CONCLUSIONS

L-T4 suppresses LPO, 2) MEL, AMB and NAC protect against LPO, 3) PTU is an antioxidant in thyreotoxicosis, however, when administered alone, it enhances LPO, 4) stress accelerate LPO.

摘要

目的

研究抗氧化剂在甲状腺毒症中的可能作用。我们检测了雄性Wistar大鼠肺匀浆中脂质过氧化（LPO）的参数：共轭二烯（CD）、丙二醛（MDA）、席夫碱（SB）。

方法

设立两个对照组：第1组——未处理动物；第2组——注射0.9%氯化钠的动物。在实验I中，动物腹腔注射L-甲状腺素（LT4）（第3 - 7组）。一周后，大鼠额外接受以下处理：第4组——褪黑素（MEL）；第5组——丙硫氧嘧啶（PTU）；第6组——氨溴索（AMB）；第7组——N-乙酰半胱氨酸（NAC）。在实验II中，动物仅接受抗氧化剂。

结果

在实验I中，我们注意到第2组的MDA和SB水平显著高于第1组。此外，我们观察到第3组的MDA和SB水平显著高于第1组，但第3组的SB水平低于第2组。与第3组相比，褪黑素、PTU和NAC降低了CD；PTU、AMB降低了MDA，MEL、AMB降低了SB水平。在实验II中，我们观察到第2组的MDA和SB水平显著高于第1组。与第2组相比，褪黑素、AMB和NAC降低了MDA和SB水平，但PTU使MDA和SB水平相对于第1组升高。