Department of Chemistry, East China Normal University, Shanghai 200062, China.
Talanta. 2010 Mar 15;80(5):1643-7. doi: 10.1016/j.talanta.2009.09.060. Epub 2009 Oct 4.
A solid-state electrochemiluminescence (ECL) biosensing switch based on special ferrocene-labeled molecular beacon (Fc-MB) has been successfully developed for T4 DNA ligase detection. Such special switch system consisted of two main parts, an ECL substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)(3)(2+)-AuNPs) onto Au electrode. A molecular beacon labeled by ferrocene as the ECL intensity switch. The molecular beacon is designed with special base sequence, which could combine with its target biomolecule via the reaction of the repair and recombination of nucleic acids by DNA ligase. During the reaction, the molecular beacon opened its stem-loop, and the labeled Fc was consequently kept away from the ECL substrate. Such structural change resulted in an obvious increment in ECL intensity due to the decreased Fc quenching effect to the ECL substrate. The analysis results are sensitive and specific.
基于特殊的二茂铁标记分子信标（Fc-MB）的固态电化学发光（ECL）生物传感开关已成功开发用于 T4 DNA 连接酶检测。这种特殊的开关系统由两个主要部分组成，一个是 ECL 基底，另一个是 ECL 强度开关。ECL 基底是通过将金纳米粒子和钌（II）三（联吡啶）（Ru（bpy）（3）（2+）-AuNPs）复合物修饰到 Au 电极上制成的。一个以二茂铁标记的分子信标作为 ECL 强度开关。分子信标设计有特殊的碱基序列，可以通过 DNA 连接酶对核酸的修复和重组反应与靶生物分子结合。在反应过程中，分子信标打开其茎环结构，标记的 Fc 因此远离 ECL 基底。这种结构变化导致 ECL 强度明显增加，因为 Fc 对 ECL 基底的猝灭效应降低。分析结果具有较高的灵敏度和特异性。
